Ribonuclease H2 subunit A Antibodies

Ribonuclease H2 subunit A (RNASEH2A)

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the elimination of lagging- strand Okazaki fragment RNA primers during DNA replication.

Mediates the excision of single ribonucleotides from DNA:RNA duplexes. .

Gene Information

Human
Gene Name:	RNASEH2A
Uniprot:	O75792
Entrez:	10535

Family

Belongs to:
RNase HII family

Alternative Names

AGS4RNase H2 subunit A; Aicardi-Goutieres syndrome 4 protein; Aicardi-Goutieres syndrome 4; JUNB; ribonuclease H2 subunit A; ribonuclease H2, large subunit; ribonuclease H2, subunit A; Ribonuclease HI large subunit; Ribonuclease HI subunit A; ribonuclease HI, large subunit; RNase H(35); RNASEHIEC 3.1.26.4; RNHIARNase HI large subunit; RNHL

Molecular Weight

Mass (kDA):
33.395 kDA

Genomic Context

Human
Location:	19p13.13
Sequence:	19; NC_000019.10 (12802054..12813645)

Subcellular Localizations

Nucleus.

Diseases

• Virus Diseases
• Malignant Neoplasms
• Neoplasms
• Congenital Infectious Disease
• Neoplasm Metastasis
• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Breast
• Congenital Neurologic Anomalies
• Nervous System Disorder
• Autoimmune Diseases Of The Nervous System
• Chilblains
• Sarcoma

• Lymphocytosis
• Carcinoma
• Immunologic Deficiency Syndromes
• Encephalopathies
• Hiv Infections
• Mammary Neoplasms

Pathways

• Cell Proliferation
• Cell Cycle
• S Phase
• Pathogenesis
• Viral Replication

PTMs

• Acetylation

• Phosphoprotein

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNASEH2A

Best Uses Of The RNASEH2A Marker

Boster Bio founder Steven Boster is well-known for his high-affinity primary antibody development. His proprietary product is the RNASEH2A marker. High-affinity primary antibodies are useful in the research of RNA-dependent DNA repair and apoptosis. The RNASEH2A marker is a gene that regulates apoptosis.

High-affinity primary antibodies

There are many benefits to using RNASEH2A to detect high-affinity prim antibodies. One of these benefits is the ability identify antibodies that recognize specific antigens, and detect them in real time. The RNASEH2A mark is widely used for antibody research. The antibody is designed to target a specific antigen, which is expressed in human fibroblasts.

The RNASEH2A markers are useful tools for identifying immunoglobulins present in murine sera. This marker allows the detection of immunoglobulins on mouse HEp-2 slides. Anti-Insulin IgM in mouse sera was detected in immunized and non-immunized mice. The stained HEp-2 slide slides were analyzed with DMi8 or Axioskop 2

For the survival of transformed B cell, the IgM BCR must be present. The IgD BCR does. It was found that monovalent HEL prevented the activation the IgM-class IgD BCR by complex HEL. It is possible that the IgM-class antibody polyreactivity is a prerequisite for an effective primary immune response. However, it is unknown whether IgD-deficient B cells can mature to produce NP antibodies.

The pCEP EGFP Vector contains humanised GFP (GFP). The corresponding plasmid carries a GST-tagged and non-tagged RNASEH2A subunits. These plasmids also contain an mflucI indicator cassette. Using a BD FACSJazz instrument to sort RNASEH2AKO cell lines into 96-well plates, The PCR products were used to identify clones of RNASEH2AKO cells. Sanger DNA sequencing confirmed the clones.

Using this marker can be useful in determining cellular identity and monitoring therapy. High-affinity antibodies to RNASEH2A can be used to identify specific antigens in research. The RNASEH2A protein is an essential component in human T-cell lymphoma. The marker can also provide interesting insights into the cellular origin, the clonality, as well as the natural history of the disease.

The RNASEH2A gene regulates a variety of processes within the immune system, including the secretion of antibodies. Deficient IgD can inhibit the production of antibodies against oneself, as IgD proteins are essential for a balanced immune response. The IgD -class BCR expression has been reduced in these antigen specific antigens. This suggests that IgD -deficient B cells are more likely to produce rapid primary immune responses.

Another interesting feature of the IgD BCR is the fact that it regulates the secretion of antibodies in vivo. It can sense antigen-valence. Monovalent antigens can interfere with the production IgG. However, polyvalent antigens stimulate IgG memories in activated mature B cell. IgD-mediated regulation is essential for maturation of antibody responses and tolerance towards self-structures.

The RNASEH2A antibodies recognize human RNAse H2A. They recognize a 17-amino peptide that is located near the middle of the protein. The SoF mutant shows reduced RNase H activity in vivo against RNA-DNA heteroduplexes. These results are promising for the study of human RNA-sequencing, as they enable accurate detection of specific RNAs.

RNASEH2A - marker

Boster Bio RNASEH2A is a gene-marker kit that contains a single antibody for the detection this RNA splice variant within eukaryotes. This kit contains high-affinity, primary antibodies that are frequently cited in research. The RNAseH2A protein is highly specific for this cellular RNA variant and has been validated on Western Blotting, Immunohistochemistry, and ELISA.