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Product Info Summary
| SKU: | PB9748 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-TREX1 Antibody Picoband®
SKU/Catalog Number
PB9748
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-TREX1 Antibody Picoband® catalog # PB9748. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TREX1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9748)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human TREX1, different from the related mouse sequence by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9748 is reactive to TREX1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
38923 MW
Background of TREX1
Three prime repair exonuclease 1 is an enzyme that in humans is encoded by the TREX1 gene. This gene encodes a nuclear protein with 3' exonuclease activity. The encoded protein may play a role in DNA repair and serve as a proofreading function for DNA polymerase. It is also a component of the SET complex, and acts to rapidly degrade 3' ends of nicked DNA during granzyme A-mediated cell death. Mutations in this gene result in Aicardi-Goutieres syndrome, chilblain lupus, Cree encephalitis, and other diseases of the immune system. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9748 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of TREX1 using anti-TREX1 antibody (PB9748).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: SMMC Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TREX1 antigen affinity purified polyclonal antibody (Catalog # PB9748) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TREX1 at approximately 33 kDa. The expected band size for TREX1 is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TREX1 (Source: Uniprot.org, NCBI)
Gene Name
TREX1
Full Name
Three-prime repair exonuclease 1
Weight
38923 MW
Superfamily
exonuclease superfamily
Alternative Names
Three-prime repair exonuclease 1;3.1.11.2;3'-5' exonuclease TREX1;DNase III;TREX1; TREX1 AGS1, CRV, DRN3, HERNS, RVCLS three prime repair exonuclease 1 three-prime repair exonuclease 1|3 repair exonuclease 1|3-5 exonuclease TREX1|DNase III|deoxyribonuclease III
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TREX1, check out the TREX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TREX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TREX1 Antibody Picoband® (PB9748)
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Customer Q&As
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1 Customer Q&As for Anti-TREX1 Antibody Picoband®
Question
We are currently using anti-TREX1 antibody PB9748 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
The anti-TREX1 antibody (PB9748) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-23
