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Product Info Summary
| SKU: | M01209 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01209
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody catalog # M01209. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01209)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AEDC-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RING2 / RING1B / RNF2 E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01209 is reactive to RNF2 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
123799 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01209 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: HeLa cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of RING2 / RING1B / RNF2 expression in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For RNF2 (Source: Uniprot.org, NCBI)
Gene Name
RNF2
Full Name
E3 ubiquitin-protein ligase RING2
Weight
123799 MW
Alternative Names
Vinculin;Metavinculin;MV;VCL; RNF2 BAP-1, BAP1, DING, HIPI3, RING1B, RING2 ring finger protein 2 E3 ubiquitin-protein ligase RING2|HIP2-interacting protein 3|RING finger protein 1B|RING finger protein BAP-1|RING-type E3 ubiquitin transferase RING2|huntingtin-interacting protein 2-interacting protein 3|protein DinG
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RNF2, check out the RNF2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RNF2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody (M01209)
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Customer Q&As
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1 Customer Q&As for Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01209?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-RING2 / RING1B / RNF2 Rabbit Monoclonal Antibody (M01209) expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-10
