RNF2 Antibodies

E3 ubiquitin-protein ligase RING2 (RNF2)

H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation (By similarity).

Essential element of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class necessary to keep the transcriptionally repressive state of several genes, including Hox genes, throughout development (PubMed:16359901, PubMed:26151332). PcG PRC1 complex acts through chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:26151332).

Gene Information

Human
Gene Name:	RNF2
Uniprot:	Q99496
Entrez:	6045

Family

Belongs to:
No superfamily

Alternative Names

BAP-1; BAP1RING finger protein 1B; DING; DINGRING finger protein BAP-1; EC 6.3.2; HIPI3; HIPI3RING1BE3 ubiquitin-protein ligase RING2; Huntingtin-interacting protein 2-interacting protein 3; Protein DinG; ring finger protein 2HIP2-interacting protein 3; RING1B; RING2; RING2EC 6.3.2.-; RNF2

Molecular Weight

Mass (kDA):
37.655 kDA

Genomic Context

Human
Location:	1q25.3
Sequence:	1; NC_000001.11 (185045430..185102603)

Subcellular Localizations

Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells.

Diseases

• Malignant Neoplasms
• Neoplasms
• Lymphoma
• Cell Transformation, Neoplastic
• Atherosclerosis
• Hiv Infections
• Malignant Neoplasm Of Breast
• Cachexia
• Mammary Neoplasms
• Rheumatoid Arthritis
• Malignant Paraganglionic Neoplasm
• Atrophy
• Arthritis

• Leukemia
• Hyperplasia
• Sclerosis
• Adenoma
• Congenital Musculoskeletal Anomalies
• Ataxia
• Glioma

Interacting Proteins

• ABCB1
• BMI1
• CBX4
• CBX6
• CBX7

• CBX8
• CSNK2A1
• CSNK2B
• HIST1H2AG
• KAT8

• PCGF1
• PCGF2
• PCGF3
• PCGF5
• PCGF6

• RING1
• RYBP
• UBE2D1
• UBE2D2
• UBE2D3

• UBE2D4
• UBE2E2
• UBE2E3
• USP11
• USP7

Pathways

• Gene Silencing
• Cell Cycle
• Cell Proliferation
• Localization
• Methylation
• Cell Differentiation
• Histone Ubiquitination
• Regulation Of Gene Expression
• Chromatin Remodeling
• Histone Deacetylation
• Dna Repair
• Dna Methylation
• Pathogenesis

• Rna Interference
• Transport
• Neurogenesis
• Histone Modification
• Nuclear Export
• Chromosome Segregation
• Gliogenesis

PTMs

• Ubiquitination
• Methylation
• Phosphorylation
• Acetylation
• Deacetylation

• Demethylation
• Biotinylation
• Cleavage
• Dephosphorylation
• Deglycosylation

Research Areas

• Zinc Finger

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/RNF2

Best Uses Of The RNF2 Marker In Rabbit And Mouse Studies

You might be curious about antibodies and what to use for rabbit and mouse studies. This article will explain how to select the appropriate antibodies for your research needs. You might also be interested in learning about boster-bio antibodies. You will also learn how to use mouse and rabbit antibodies in your research. The antibodies can be used to refine your experiments.

Antibodies

An antibody that targets RNF2 may be beneficial in colorectal cancer treatment. It is able to react with both mouse and human samples and may also be useful in prognostication or therapy. It has also been proven to be involved in the development polycomb repressive complex 1, (PDCR1), that promotes metastasis of double negative prostate cancer.

Primary antibodies are for research purposes only. They have not been approved for clinical diagnosis. The antibodies used for this study are available at CUSABIO BIOTEC Co. Ltd. Wuhan. They can be used for up 1 year. This research has been published and supported by the National Cancer Institute in a number of peer-reviewed journals. Please contact the company if the RNF2 marker is used to make an antibody.

No clinical trials have yet been conducted to investigate the role of antibodies that are against the RNF2 gene. Although research is ongoing on this topic, it has been shown that it is a promising biomarker in this condition. It has also been shown that it is associated with the patient's stage or differentiation status. In other words, there is a correlation between RNF2 expression and survival. Further studies are warranted to determine if this is a reliable marker for predicting patient outcomes.

RNF2 expression has been examined in normal skin and melanoma. RNF2 expression was higher in tumor cells, in both types and types of melanoma. This enhances the proliferation of tumor cells and also contributes to metastasis in immunodeficient mice. RNF2 intratumoral expressing the gene is associated for improved survival.

A knockdown of RNF2 was done to determine the role of RNF2 for tumor growth. RNF2 gene knockdown resulted a higher accumulation of RASSF10 proteins. In addition, RNF2 was knocked down in cancer cells. This resulted to a decrease in RNF10. The RNF2 gene is expressed in antibodies that are produced in mice. This makes them more effective in tumor-targeting studies.

Mouse

There are a few important considerations to be aware of when using RNF2 for your marker. RNF1 has an immunogenicity that is quite high, while RNF2 is significantly lower. This marker is currently being tested in clinical trials. These are the most common uses for the RNF2 mark. Below are some examples.

Rabbit

RNF2 expression in cancers is related to stage and differentiation status. We have shown that RNF2 expression in HCC patients is at a protein level. Interestingly, the expression of RNF2 in tumors in mice and rabbits was unaffected by loss of RNF2.

Monoclonal monoclonal rabbit antibody, the RING1B XP(r), is able to recognize endogenous levels. The recombinant Protein was immunized to produce the mAb. The antibody binds to the culture media's endogenous levels RING1B protein. The animal was imaged and genotyped in order to visualize the antibody reaction.

For immunoprecipitation, we used 2mL of 35S-labeled sik1 cells in a reaction solution containing 50mM Tris*HCl at pH 7.5, and 10mg of ubiquitin. Two mLs SIK1 was mixed with 2mg of RNF2 and were incubated in this solution for two hours. The reaction mixture of SIK1 and RNF2 was separated by SDS/PAGE. It was then probed with the indicated antibody.

Mammalian heart development is affected by the RNF2 Protein. Some studies have shown that the RNF2 protein is essential for tissue integrity. This list is augmented by data from Zebrafish on rnf2 mutations. This protein is partially responsible for controlling the conductive pathway. It regulates the conductive cell identities and chamber. Cardiac defects can result from disruptions in RNF2 function.