Product Info Summary
SKU: | A11058 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-TUBGCP4/Gcp4 Antibody
SKU/Catalog Number
A11058
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-TUBGCP4/Gcp4 Antibody (Catalog # A11058). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TUBGCP4/Gcp4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11058)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human TUBGCP4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11058 is reactive to TUBGCP4 in Human, Mouse
Reconstitution
Calculated molecular weight
76089 MW
Background of GCP4
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Fava F., J. Cell Biol. 147:857-868(1999).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11058 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from COLO cells and HT-29 cells, using TUBGCP4 antibody A11058.
Protein Target Info & Infographic
Gene/Protein Information For TUBGCP4 (Source: Uniprot.org, NCBI)
Gene Name
TUBGCP4
Full Name
Gamma-tubulin complex component 4
Weight
76089 MW
Superfamily
TUBGCP family
Alternative Names
Gamma-tubulin complex component 4;GCP-4;hGCP4;Gamma-ring complex protein 76 kDa;h76p;hGrip76;TUBGCP4;76P, GCP4; TUBGCP4 76P, GCP-4, GCP4, Grip76, MCCRP3 tubulin gamma complex associated protein 4 gamma-tubulin complex component 4|gamma tubulin ring complex protein (76p gene)|gamma-ring complex protein 76 kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBGCP4, check out the TUBGCP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBGCP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TUBGCP4/Gcp4 Antibody (A11058)
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No publications found for A11058
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-TUBGCP4/Gcp4 Antibody
Question
Would anti-TUBGCP4/Gcp4 antibody A11058 work for WB with neuroblastoma?
O. Anderson
Verified customer
Asked: 2019-10-22
Answer
According to the expression profile of neuroblastoma, TUBGCP4 is highly expressed in neuroblastoma. So, it is likely that anti-TUBGCP4/Gcp4 antibody A11058 will work for WB with neuroblastoma.
Boster Scientific Support
Answered: 2019-10-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neuroblastoma using anti-TUBGCP4/Gcp4 antibody A11058. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-08
Question
I was wanting to use your anti-TUBGCP4/Gcp4 antibody for WB for human neuroblastoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human neuroblastoma identification?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
As indicated on the product datasheet, A11058 anti-TUBGCP4/Gcp4 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human neuroblastoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-27
Question
I see that the anti-TUBGCP4/Gcp4 antibody A11058 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-13
Question
I have a question about product A11058, anti-TUBGCP4/Gcp4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11058 anti-TUBGCP4/Gcp4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-23
Question
Please see the WB image, lot number and protocol we used for neuroblastoma using anti-TUBGCP4/Gcp4 antibody A11058. Please let me know if you require anything else.
J. Patel
Verified customer
Asked: 2019-03-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-03-19
Question
I am interested in to test anti-TUBGCP4/Gcp4 antibody A11058 on human neuroblastoma for research purposes, then I may be interested in using anti-TUBGCP4/Gcp4 antibody A11058 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-02-18
Answer
The products we sell, including anti-TUBGCP4/Gcp4 antibody A11058, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-02-18