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Product Info Summary
| SKU: | M00207-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Rho A + B + C Rabbit Monoclonal Antibody
SKU/Catalog Number
M00207-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Rho A + B + C Rabbit Monoclonal Antibody catalog # M00207-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Rho A + B + C Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DGF-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Rho A + B + C
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00207-1 is reactive to RHOA in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
22 kDa
Calculated molecular weight
22 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00207-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:500-1:1000
ICC/IF 1:100-1:500
FC 1:200
Positive Control
WB: human HUVEC whole cell, human THP-1 whole cell, human Hela whole cell, human U251 whole cell, rat brain tissue, rat lung tissue, mouse brain tissue, mouse lung tissue
IHC: human lung carcinoma tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Rho A+B+C using anti-Rho A+B+C antibody (M00207-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HUVEC whole cell lysates,
Lane 2: human THP-1 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: human U251 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Rho A+B+C antigen affinity purified monoclonal antibody (Catalog # M00207-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Rho A+B+C at approximately 22 kDa. The expected band size for Rho A+B+C is at 22 kDa.
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Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody .
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Immunofluorescent analysis of Hela cells, using Rho A + B + C Antibody .
Protein Target Info & Infographic
Gene/Protein Information For RHOA (Source: Uniprot.org, NCBI)
Gene Name
RHOA
Full Name
Transforming protein RhoA
Weight
22 kDa
Superfamily
small GTPase superfamily
Alternative Names
Transforming protein RhoA;Rho cDNA clone 12;h12;RHOA;ARH12, ARHA, RHO12; RHOA ARH12, ARHA, EDFAOB, RHO12, RHOH12 ras homolog family member A transforming protein RhoA|Aplysia ras-related homolog 12|epididymis secretory sperm binding protein|oncogene RHO H12|small GTP binding protein RhoA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RHOA, check out the RHOA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RHOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Rho A + B + C Rabbit Monoclonal Antibody (M00207-1)
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