Anti-RHOA

Product Name

Anti-RHOA

View all RHOA Antibodies

SKU/Catalog Number

M00207-3

Size

50 µl

Description

Boster Bio Anti-RHOA (Catalog # M00207-3). Tested in WB application(s). This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-RHOA (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00207-3)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

2148CT124.4.9

Isotype

IgG1,κ

Immunogen

This antibody is generated from a mouse immunized with a recombinant protein from human.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00207-3 is reactive to RHOA in Human, Mouse, Rat

Applications

M00207-3 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

21768 Da

Background of RHOA

Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. Stimulates PKN2 kinase activity. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Regulates a signal transduction pathway linking plasma membrane receptors to the assembly of focal adhesions and actin stress fibers. Involved in a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Plays an essential role in cleavage furrow formation. Required for the apical junction formation of keratinocyte cell-cell adhesion. May be an activator of PLCE1. Activated by ARHGEF2, which promotes the exchange of GDP for GTP. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization (By similarity). Regulates KCNA2 potassium channel activity by reducing its location at the cell surface in response to CHRM1 activation; promotes KCNA2 endocytosis (PubMed:9635436, PubMed:19403695).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1：160000

Validation Images & Assay Conditions

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All lanes : Anti-RHOA at dilution
Lane 1: PC-12 whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: L929 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 22 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For RHOA (Source: Uniprot.org, NCBI)

Alternative Names

h12; oncogene RHO H12; ras homolog gene family, member A; Rho cDNA clone 12; RHO12; RhoA; RHOH12ARH12ARHAAplysia ras-related homolog 12; small GTP binding protein RhoA; transforming protein RhoA RHOA ARH12, ARHA, EDFAOB, RHO12, RHOH12 ras homolog family member A transforming protein RhoA|Aplysia ras-related homolog 12|epididymis secretory sperm binding protein|oncogene RHO H12|small GTP binding protein RhoA

For more info on RHOA, check out the RHOA Infographic

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In this infographic, you will see the following information for RHOA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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