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Product Info Summary
| SKU: | A30447 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-RFA2 Antibody
SKU/Catalog Number
A30447
Size
100ul
Form
Liquid
Description
Boster Bio Anti-RFA2 Antibody (Catalog# A30447). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, .
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RFA2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30447)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human RFA2. Immunogen sequence location: 10-59
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30447 is reactive to RPA2 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
29247 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30447 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from K562 cells, using RFA2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of HeLa cells, using RFA2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For RPA2 (Source: Uniprot.org, NCBI)
Gene Name
RPA2
Full Name
Replication protein A 32 kDa subunit
Weight
29247 MW
Superfamily
replication factor A protein 2 family
Alternative Names
RPA2;REPA2;RPA32;RPA34;Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34 RPA2 REPA2, RP-A p32, RP-A p34, RPA32 replication protein A2 replication protein A 32 kDa subunit|RF-A protein 2|replication factor A protein 2|replication protein A 34 kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPA2, check out the RPA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RFA2 Antibody (A30447)
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No publications found for A30447
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Customer Q&As
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4 Customer Q&As for Anti-RFA2 Antibody
Question
Is a blocking peptide available for product anti-RFA2 antibody (A30447)?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do provide the blocking peptide for product anti-RFA2 antibody (A30447). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-28
Question
I was wanting to use to test anti-RFA2 antibody A30447 on human cervix carcinoma for research purposes, then I may be interested in using anti-RFA2 antibody A30447 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Parker
Verified customer
Asked: 2019-01-21
Answer
The products we sell, including anti-RFA2 antibody A30447, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-21
Question
I see that the anti-RFA2 antibody A30447 works with IF, what is the protocol used to produce the result images on the product page?
Z. Parker
Verified customer
Asked: 2014-04-08
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2014-04-08
Question
Is this A30447 anti-RFA2 antibody reactive to the isotypes of RPA2?
M. Jha
Verified customer
Asked: 2014-01-07
Answer
The immunogen of A30447 anti-RFA2 antibody is A peptide derived from human RFA2. Immunogen sequence location: 10-59. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-01-07
