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Product Info Summary
| SKU: | A01882-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-Kallikrein 6/KLK6 Antibody Picoband®
View all Kallikrein 6/Neurosin Antibodies
SKU/Catalog Number
A01882-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 6/KLK6 Antibody Picoband® catalog # A01882-2. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 6/KLK6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01882-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Kallikrein 6 recombinant protein (Position: L22-K244).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01882-2 is reactive to KLK6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
29 kDa
Calculated molecular weight
26.856kDa
Background of Kallikrein 6/Neurosin
KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01882-2 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Kallikrein 6/KLK6 using anti-Kallikrein 6/KLK6 antibody (A01882-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 6/KLK6 antigen affinity purified polyclonal antibody (Catalog # A01882-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 6/KLK6 at approximately 29 kDa. The expected band size for Kallikrein 6/KLK6 is at 27 kDa.
Protein Target Info & Infographic
Gene/Protein Information For KLK6 (Source: Uniprot.org, NCBI)
Gene Name
KLK6
Full Name
Kallikrein-6
Weight
26.856kDa
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-6; Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme; KLK6; PRSS18; PRSS9 KLK6 Bssp, Klk7, PRSS18, PRSS9, SP59, hK6 kallikrein related peptidase 6 kallikrein-6|neurosin|protease M|serine protease 18|serine protease 9|zyme
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK6, check out the KLK6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 6/KLK6 Antibody Picoband® (A01882-2)
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