Click image to see more details
Product Info Summary
| SKU: | P02067-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody
SKU/Catalog Number
P02067-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody catalog # P02067-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P02067-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
FCG-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Phospho-RPA2 (T21)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P02067-1 is reactive to RPA2 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
29 kDa
Calculated molecular weight
29 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P02067-1 is guaranteed for IP, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:5000-1:10000
IP 1:20
Positive Control
WB: human HepG2 whole cell, human Jurkat whole cell, human 293T whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RPA2 using anti-RPA2 antibody (P02067-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPA2 antigen affinity purified monoclonal antibody (Catalog # P02067-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPA2 at approximately 29 kDa. The expected band size for RPA2 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For RPA2 (Source: Uniprot.org, NCBI)
Gene Name
RPA2
Full Name
Replication protein A 32 kDa subunit
Weight
29 kDa
Superfamily
replication factor A protein 2 family
Alternative Names
Replication protein A 32 kDa subunit;RP-A p32;Replication factor A protein 2;RF-A protein 2;Replication protein A 34 kDa subunit;RP-A p34;RPA2;REPA2, RPA32, RPA34; RPA2 REPA2, RP-A p32, RP-A p34, RPA32 replication protein A2 replication protein A 32 kDa subunit|RF-A protein 2|replication factor A protein 2|replication protein A 34 kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RPA2, check out the RPA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RPA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody (P02067-1)
Hello CJ!
No publications found for P02067-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-Phospho-RPA2 (T21) Rabbit Monoclonal Antibody
Question
We are currently using anti-Phospho-RPA2 (T21) Rabbit Monoclonal antibody P02067-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The anti-Phospho-RPA2 (T21) Rabbit Monoclonal antibody (P02067-1) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-02
