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Product Info Summary
| SKU: | A03004-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RASGRP1 Antibody Picoband®
SKU/Catalog Number
A03004-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RASGRP1 Antibody Picoband® catalog # A03004-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RASGRP1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03004-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human RASGRP1 recombinant protein (Position: R8-Q678).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03004-1 is reactive to RASGRP1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
100 kDa
Calculated molecular weight
90.402kDa
Background of RASGRP1
RAS guanyl-releasing protein 1 is a protein that in humans is encoded by the RASGRP1 gene. This gene is a member of a family of genes characterized by the presence of a Ras superfamily guanine nucleotide exchange factor (GEF) domain. It functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound GDP for GTP. It activates the Erk/MAP kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation. Alternatively spliced transcript variants encoding different isoforms have been identified. Altered expression of the different isoforms of this protein may be a cause of susceptibility to systemic lupus erythematosus (SLE).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03004-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human Jurkat whole cell, human 293T whole cell, human U-87MG whole cell
FCM: MCF-7 cell, U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of RASGRP1 using anti-RASGRP1 antibody (A03004-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human U-87MG whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RASGRP1 antigen affinity purified polyclonal antibody (Catalog # A03004-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RASGRP1 at approximately 100 kDa. The expected band size for RASGRP1 is at 90 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-RASGRP1 antibody (A03004-1).
Overlay histogram showing MCF-7 cells stained with A03004-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASGRP1 Antibody (A03004-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 3. Flow Cytometry analysis of U2OS cells using anti-RASGRP1 antibody (A03004-1).
Overlay histogram showing U2OS cells stained with A03004-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RASGRP1 Antibody (A03004-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For RASGRP1 (Source: Uniprot.org, NCBI)
Gene Name
RASGRP1
Full Name
RAS guanyl-releasing protein 1
Weight
90.402kDa
Superfamily
RASGRP family
Alternative Names
Endoglin; Cell surface MJ7/18 antigen; CD105; Eng; Edg; RASGRP1 CALDAG-GEFI, CALDAG-GEFII, IMD64, RASGRP RAS guanyl releasing protein 1 RAS guanyl-releasing protein 1|RAS guanyl nucleotide-releasing protein 1|RAS guanyl releasing protein 1 (calcium and DAG-regulated)|calcium and DAG-regulated guanine nucleotide exchange factor II|calcium- and diacylglycerol-regulated guanine nucleotide exchange factor II|guanine nucleotide exchange factor, calcium- and DAG-regulated, Rap1A|ras activator RasGRP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RASGRP1, check out the RASGRP1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RASGRP1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RASGRP1 Antibody Picoband® (A03004-1)
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