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Product Info Summary
| SKU: | RP1097 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-PU.1/Spi1 Antibody
View all PU.1/Spi-1 Antibodies
SKU/Catalog Number
RP1097
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PU.1/Spi1 Antibody catalog # RP1097. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PU.1/Spi1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1097)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PU.1/Spi1 recombinant protein (Position: E18-K196). Human PU.1/Spi1 shares 84% and 84.4% amino acid (aa) sequence identity with mouse and rat PU.1/Spi1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1097 is reactive to SPI1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
150 kDa
Calculated molecular weight
31083 MW
Background of PU.1/Spi-1
Transcription factor PU.1 is a protein that in humans is encoded by the SPI1 gene. This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1097 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Positive Control
IHC: Mouse Spleen Tissue, Rat Spleen Tissue, Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-PU.1/Spi1 antibody, RP1097, IHC(P)
IHC(P): Mouse Spleen Tissue
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Anti-PU.1/Spi1 antibody, RP1097, IHC(P)
IHC(P): Rat Spleen Tissue
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Anti-PU.1/Spi1 antibody, RP1097, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For SPI1 (Source: Uniprot.org, NCBI)
Gene Name
SPI1
Full Name
Transcription factor PU.1
Weight
31083 MW
Superfamily
ETS family
Alternative Names
Transcription factor PU.1;31 kDa-transforming protein;SPI1; SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A Spi-1 proto-oncogene transcription factor PU.1|31 kDa transforming protein|hematopoietic transcription factor PU.1|spleen focus forming virus (SFFV) proviral integration oncogene spi1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SPI1, check out the SPI1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SPI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PU.1/Spi1 Antibody (RP1097)
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No publications found for RP1097
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Customer Q&As
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1 Customer Q&As for Anti-PU.1/Spi1 Antibody
Question
We are currently using anti-PU.1/Spi1 antibody RP1097 for rat tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on bovine tissues as well?
S. Collins
Verified customer
Asked: 2014-06-16
Answer
The anti-PU.1/Spi1 antibody (RP1097) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-06-16
