Anti-SPI1 Antibody

Product Name

Anti-SPI1 Antibody

View all PU.1/Spi-1 Antibodies

SKU/Catalog Number

M01116-1

Size

50 µl

Description

Boster Bio Anti-SPI1 Antibody (Catalog # M01116-1). Tested in WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-SPI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01116-1)

Host

Mouse

Contents

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Monoclonal

Clone Number

1835CT291.10.52

Isotype

IgG1,k

Immunogen

This SPI1 antibody is generated from a mouse immunized with a recombinant protein of human SPI1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01116-1 is reactive to SPI1 in Human

Applications

M01116-1 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

31083 Da

Background of PU.1/Spi-1

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing (By similarity).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:1000

Validation Images & Assay Conditions

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All lanes : Anti-SPI1 Antibody at 1:500-1:1000 dilution
Lane 1: K562 whole cell lysate
Lane 2: Daudi whole cell lysate
Lane 3: THP-1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 31 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For SPI1 (Source: Uniprot.org, NCBI)

Alternative Names

hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SFPI131 kDa transforming protein; SPI-1 proto-oncogene; SPI1; Spi-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein; transcription factor PU.1 SPI1 OF, PU.1, SFPI1, SPI-1, SPI-A Spi-1 proto-oncogene transcription factor PU.1|31 kDa transforming protein|hematopoietic transcription factor PU.1|spleen focus forming virus (SFFV) proviral integration oncogene spi1

For more info on SPI1, check out the SPI1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SPI1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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