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Product Info Summary
| SKU: | PA2046 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PKM2 Antibody Picoband®
SKU/Catalog Number
PA2046
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PKM2 Antibody catalog # PA2046. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PKM2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2046)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2046 is reactive to PKM in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
57937 MW
Background of PKM
PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25.Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2046 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human A549 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PKM2 using anti-PKM2 antibody (PA2046).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM2 antigen affinity purified polyclonal antibody (Catalog # PA2046) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PKM2 at approximately 58 kDa. The expected band size for PKM2 is at 58 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PKM (Source: Uniprot.org, NCBI)
Gene Name
PKM
Full Name
Pyruvate kinase PKM
Weight
57937 MW
Superfamily
pyruvate kinase family
Alternative Names
Pyruvate kinase PKM;2.7.1.40;Cytosolic thyroid hormone-binding protein;CTHBP;Opa-interacting protein 3;OIP-3;Pyruvate kinase 2/3;Pyruvate kinase muscle isozyme;Thyroid hormone-binding protein 1;THBP1;Tumor M2-PK;p58;PKM;OIP3, PK2, PK3, PKM2; PKM CTHBP, HEL-S-30, OIP3, PK32, TCB, THBP1, p58, PKM pyruvate kinase M1/2 pyruvate kinase PKM|OPA-interacting protein 3|PK, muscle type|cytosolic thyroid hormone-binding protein|epididymis secretory protein Li 30|pyruvate kinase 2/3|pyruvate kinase isozymes M1/M2|pyruvate kinase muscle isozyme|pyruvate kinase, muscle|thyroid hormone-binding protein 1|thyroid hormone-binding protein, cytosolic|tumor M2-PK
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PKM, check out the PKM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PKM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PKM2 Antibody Picoband® (PA2046)
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Customer Q&As
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5 Customer Q&As for Anti-PKM2 Antibody Picoband®
Question
Here is the WB image, lot number and protocol we used for retina rhabdomyosarcoma using anti-PKM2 antibody PA2046. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-26
Question
Would anti-PKM2 antibody PA2046 work for WB with retina rhabdomyosarcoma?
Verified Customer
Verified customer
Asked: 2020-02-25
Answer
According to the expression profile of retina rhabdomyosarcoma, PKM is highly expressed in retina rhabdomyosarcoma. So, it is likely that anti-PKM2 antibody PA2046 will work for WB with retina rhabdomyosarcoma.
Boster Scientific Support
Answered: 2020-02-25
Question
you antibody to test anti-PKM2 antibody PA2046 on rat retina rhabdomyosarcoma for research purposes, then I may be interested in using anti-PKM2 antibody PA2046 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The products we sell, including anti-PKM2 antibody PA2046, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-28
Question
Does PA2046 anti-PKM2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-08-24
Answer
It shows on the product datasheet, PA2046 anti-PKM2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-08-24
Question
Is a blocking peptide available for product anti-PKM2 antibody (PA2046)?
Verified Customer
Verified customer
Asked: 2017-10-17
Answer
We do provide the blocking peptide for product anti-PKM2 antibody (PA2046). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-17
