Transcription factor PU.1 Antibodies

AND SPI1 ELISA kits, Transcription factor PU.1 Proteins

Many biological assays use antibodies to detect Transcription factor PU.1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Transcription factor PU.1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Transcription factor PU.1 antibodies...

We validate the specificity of these antibodies to Transcription factor PU.1 by testing them on tissues known to express SPI1 positively and negatively. Browse below to find the SPI1 antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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SPI1 Infographic by Boster Bio

All SPI1 Products

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Product

Figures

Specificity

Applications

Price

Anti-PU.1/Spi1 Antibody

1 Q&As

Human, Mouse, Rat

IHC

$370
Cat # RP1097
100 μg/vial

Anti-SPI1 Rabbit Monoclonal Antibody

5 Q&As

Human

Flow Cytometry, IHC, WB

$370
Cat # M01116
100 μl

SPI1 Colorimetric Cell-Based ELISA

Human, Mouse, Rat

ELISA

$480
Cat # EKC1684
1 kit, containing one 96-well plate and all necessary reagents

Anti-PU.1 (R212) SPI1 Antibody

Human, Mouse, Rat

IF, IHC, WB

$405
Cat # A01116
100ul

Human SPI1 Recombinant Protein

SDS-PAGE, Denatured

$250
Cat # PROTP17947
Starting from 10ug

Anti-SPI1 Antibody

Human

$299
Cat # M01116-1
50 µl

Anti-PU.1/SPI1 Antibody Picoband®

Human, Mouse, Rat

ELISA, Flow Cytometry, IHC, WB

$370
Cat # A01116-1
100 μg/vial

SPI1 (NM_001080547) Human Over-expression Lysate

Human

$628
Cat # LS006688
100 µg

SPI1 Overview

Facts about Transcription factor PU.1.

SPI1 3D structure. Source: alphafold

Transcription factor PU.1 (SPI1)

Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells.

Also binds RNA and may modulate pre-mRNA splicing (By similarity). .

Gene Information

Human
Gene Name:	SPI1
Uniprot:	P17947
Entrez:	6688

Family

Belongs to:

ETS family

Alternative Names

hematopoietic transcription factor PU.1; OF; PU.1; SFPI1; SFPI131 kDa transforming protein; SPI-1 proto-oncogene; SPI1; Spi-1; SPI-A; spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein; transcription factor PU.1

Molecular Weight

Mass (kDA):
31.083 kDA

Genomic Context


Human

Location:	11p11.2
Sequence:	11; NC_000011.10 (47354859..47395640, complement)

Subcellular Localizations

Nucleus.

Diseases

Salmonella Infections
Cell Invasion
Leukemia
Salmonella Infections, Animal
Diarrhea
Malignant Neoplasms
Myeloid Leukemia
Inflammation
Neoplasms
Leukemia, Myelocytic, Acute
Infective Disorder
Tissue Adhesions
Disease Caused By Shigella Flexneri

Gastroenteritis
Typhoid Fever
Inflammatory Diarrhea
Systemic Infection
Shigella Infections
Yersinia Infections
Lymphoma

Interacting Proteins

GATA2
HOXA10
PRKDC

Pathways

Secretion
Virulence
Pathogenesis
Transport
Phagocytosis
Cell Cycle
Mitosis
Immune Response
Protein Secretion
Dna Replication
Cell Death
Oncogenesis
Inflorescence Development

Cell Division
Fermentation
Histone Acetylation
Chromosome Condensation
Cell Proliferation
Dna Methylation
Auxin Transport

PTMs

Phosphorylation
Acetylation
Methylation

Cleavage
Prenylation
Ribosylation

Ubiquitination

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/SPI1

References

PMID: 1693183 by Ray D., et al. The human homologue of the putative proto-oncogene Spi-1: characterization and expression in tumors.

PMID: 10196196 by Rao S., et al. SPI-B activates transcription via a unique proline, serine, and threonine domain and exhibits DNA binding affinity differences from PU.1.