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Product Info Summary
| SKU: | A05053-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PTGIS/Prostaglandin I2 Synthase Antibody
View all Prostaglandin I2 Synthase Antibodies
SKU/Catalog Number
A05053-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-PTGIS/Prostaglandin I2 Synthase Antibody (Catalog # A05053-1). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PTGIS/Prostaglandin I2 Synthase Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05053-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human PTGIS.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05053-1 is reactive to PTGIS in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
57104 MW
Background of Prostaglandin I2 Synthase
Catalyzes the isomerization of prostaglandin H2 to prostacyclin (= prostaglandin I2).
Miyata A., Biochem. Biophys. Res. Commun. 200:1728-1734(1994).
Chevalier D., Hum. Genet. 108:148-155(2001).
Deloukas P., Nature 414:865-871(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05053-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using PTGIS antibody A05053-1.
Protein Target Info & Infographic
Gene/Protein Information For PTGIS (Source: Uniprot.org, NCBI)
Gene Name
PTGIS
Full Name
Prostacyclin synthase
Weight
57104 MW
Superfamily
cytochrome P450 family
Alternative Names
Prostacyclin synthase;5.3.99.4 ;Prostaglandin I2 synthase;PTGIS;CYP8, CYP8A1; PTGIS CYP8, CYP8A1, PGIS, PTGI prostaglandin I2 synthase prostacyclin synthase|cytochrome P450, family 8, subfamily A, polypeptide 1|hydroperoxy icosatetraenoate dehydratase|prostaglandin I2 (prostacyclin) synthase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTGIS, check out the PTGIS Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTGIS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTGIS/Prostaglandin I2 Synthase Antibody (A05053-1)
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No publications found for A05053-1
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-PTGIS/Prostaglandin I2 Synthase Antibody
Question
My question regarding product A05053-1, anti-PTGIS/Prostaglandin I2 Synthase antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-03
Question
Does A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
It shows on the product datasheet, A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-27
Question
Will anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 work for WB with aorta?
Verified Customer
Verified customer
Asked: 2019-07-30
Answer
According to the expression profile of aorta, PTGIS is highly expressed in aorta. So, it is likely that anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 will work for WB with aorta.
Boster Scientific Support
Answered: 2019-07-30
Question
I see that the anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 works with WB, what is the protocol used to produce the result images on the product page?
G. Huang
Verified customer
Asked: 2019-01-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-01-11
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for aorta using anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1. Let me know if you need anything else.
D. Baker
Verified customer
Asked: 2019-01-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-10
Question
Is a blocking peptide available for product anti-PTGIS/Prostaglandin I2 Synthase antibody (A05053-1)?
Verified Customer
Verified customer
Asked: 2018-12-17
Answer
We do provide the blocking peptide for product anti-PTGIS/Prostaglandin I2 Synthase antibody (A05053-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-17
Question
Is there a BSA free version of anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 available?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-31
Question
Please see the WB image, lot number and protocol we used for aorta using anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-09-03
Question
We are currently using anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
J. Jackson
Verified customer
Asked: 2018-08-15
Answer
The anti-PTGIS/Prostaglandin I2 Synthase antibody (A05053-1) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-15
Question
I was wanting to use your anti-PTGIS/Prostaglandin I2 Synthase antibody for WB for mouse aorta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse aorta identification?
Verified Customer
Verified customer
Asked: 2018-07-09
Answer
It shows on the product datasheet, A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse aorta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-09
Question
We want to test anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 on mouse aorta for research purposes, then I may be interested in using anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-26
Answer
The products we sell, including anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-26
Question
Is this A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody reactive to the isotypes of PTGIS?
N. Jha
Verified customer
Asked: 2015-12-02
Answer
The immunogen of A05053-1 anti-PTGIS/Prostaglandin I2 Synthase antibody is Synthesized peptide derived from Internal of human PTGIS. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-12-02
Question
Will anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 work on pig WB with brain?
J. Jones
Verified customer
Asked: 2013-10-15
Answer
Our lab technicians have not validated anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-PTGIS/Prostaglandin I2 Synthase antibody A05053-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-10-15
