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Product Info Summary
| SKU: | A07761 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-eRF3a GSPT1 Antibody
SKU/Catalog Number
A07761
Size
100μl
Form
Liquid
Description
Boster Bio Anti-eRF3a GSPT1 Antibody catalog # A07761. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-eRF3a GSPT1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07761)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human eRF3a. at AA rangle: 70-150
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07761 is reactive to GSPT1 in Human, Mouse, Rat
Applications
A07761 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
55756 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of 3T3 cells using eRF3a Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For GSPT1 (Source: Uniprot.org, NCBI)
Gene Name
GSPT1
Full Name
Eukaryotic peptide chain release factor GTP-binding subunit ERF3A
Weight
55756 MW
Superfamily
TRAFAC class translation factor GTPase superfamily
Alternative Names
ERF3A; eRF3aFLJ38048; ETF3A; eukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; FLJ39067; G1 to S phase transition 1,551G9.2; G1 to S phase transition protein 1 homolog; GST1 GSPT1 551G9.2, ETF3A, GST1, eRF3a G1 to S phase transition 1 eukaryotic peptide chain release factor GTP-binding subunit ERF3A|G1 to S phase transition protein 1 homolog|eukaryotic peptide chain release factor subunit 3a|eukaryotic release factor 3a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GSPT1, check out the GSPT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GSPT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-eRF3a GSPT1 Antibody (A07761)
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No publications found for A07761
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-eRF3a GSPT1 Antibody
Question
Does anti-eRF3a antibody A07761 work on zebrafish WB with lung?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Our lab technicians have not tested anti-eRF3a antibody A07761 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-eRF3a antibody A07761 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish lung in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-20
Question
Is this A07761 anti-eRF3a antibody reactive to the isotypes of GSPT1?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The immunogen of A07761 anti-eRF3a antibody is Synthesized peptide derived from the Internal region of human eRF3a. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-15
Question
We are currently using anti-eRF3a antibody A07761 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-12
Answer
The anti-eRF3a antibody (A07761) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-12
Question
Will A07761 anti-eRF3a antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
It shows on the product datasheet, A07761 anti-eRF3a antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-22
Question
Is there a BSA free version of anti-eRF3a antibody A07761 available?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-eRF3a antibody A07761 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-eRF3a antibody A07761. Let me know if you need anything else.
C. Moore
Verified customer
Asked: 2016-05-12
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-05-12
