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Product Info Summary
| SKU: | A09870 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CLIC3 Antibody
SKU/Catalog Number
A09870
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CLIC3 Antibody (Catalog # A09870). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLIC3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09870)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CLIC3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09870 is reactive to CLIC3 in Human
Reconstitution
Calculated molecular weight
26648 MW
Background of CLIC3
Can insert into membranes and form chloride ion channels. May participate in cellular growth control.
Humphray S.J., Nature 429:369-374(2004).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Qian Z., J. Biol. Chem. 274:1621-1627(1999).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09870 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from K562 cells, using CLIC3 antibody A09870.
Protein Target Info & Infographic
Gene/Protein Information For CLIC3 (Source: Uniprot.org, NCBI)
Gene Name
CLIC3
Full Name
Chloride intracellular channel protein 3
Weight
26648 MW
Superfamily
chloride channel CLIC family
Alternative Names
Chloride intracellular channel protein 3;CLIC3; CLIC3 chloride intracellular channel 3 chloride intracellular channel protein 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLIC3, check out the CLIC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLIC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLIC3 Antibody (A09870)
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No publications found for A09870
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CLIC3 Antibody
Question
I have a question about product A09870, anti-CLIC3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A09870 anti-CLIC3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-03
Question
I see that the anti-CLIC3 antibody A09870 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-22
Question
Will anti-CLIC3 antibody A09870 work on feline WB with lower esophagus mucosa?
Verified Customer
Verified customer
Asked: 2018-07-12
Answer
Our lab technicians have not validated anti-CLIC3 antibody A09870 on feline. You can run a BLAST between feline and the immunogen sequence of anti-CLIC3 antibody A09870 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline lower esophagus mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-07-12
Question
We are currently using anti-CLIC3 antibody A09870 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
N. Parker
Verified customer
Asked: 2017-11-29
Answer
The anti-CLIC3 antibody (A09870) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-11-29
Question
I am interested in to test anti-CLIC3 antibody A09870 on human lower esophagus mucosa for research purposes, then I may be interested in using anti-CLIC3 antibody A09870 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
The products we sell, including anti-CLIC3 antibody A09870, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-08-30
Question
See attached the WB image, lot number and protocol we used for lower esophagus mucosa using anti-CLIC3 antibody A09870. Please let me know if you require anything else.
K. Yang
Verified customer
Asked: 2016-07-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-07-08
