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Product Info Summary
| SKU: | A08763-1 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-PTER Antibody Picoband®
SKU/Catalog Number
A08763-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PTER Antibody Picoband® catalog # A08763-1. Tested in ELISA, WB, applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PTER Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08763-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PTER recombinant protein (Position: M1-H315).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08763-1 is reactive to PTER in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
39 kDa
Calculated molecular weight
39.018kDa
Background of PTER
PTER is a mammalian homolog to bacterial phosphotriesterases, enzymes that hydrolyze phosphotriester-containing organophosphate pesticides. It is expressed primarily in the proximal renal tubules and the gene has been localized in humans to chromosomal band 10p12 by in situ hybridization. PTER, in addition to FTO, MC4R, and NPC1 has recently been shown to be a new risk loci for early-onset and morbid adult obesity in European populations. At least two isoforms of PTER are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08763-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PTER using anti-PTER antibody (A08763-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTER antigen affinity purified polyclonal antibody (Catalog # A08763-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTER at approximately 39 kDa. The expected band size for PTER is at 39 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PTER (Source: Uniprot.org, NCBI)
Gene Name
PTER
Full Name
Phosphotriesterase-related protein
Weight
39.018kDa
Superfamily
metallo-dependent hydrolases superfamily
Alternative Names
Epidermal growth factor-like protein 6; EGF-like protein 6; MAM and EGF domains-containing gene protein; EGFL6; MAEG; PP648; UNQ281/PRO320 PTER HPHRP, RPR-1 phosphotriesterase related phosphotriesterase-related protein|parathion hydrolase-related protein|resiniferatoxin-binding, phosphotriesterase-related
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PTER, check out the PTER Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PTER: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PTER Antibody Picoband® (A08763-1)
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