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Product Info Summary
| SKU: | PB9351 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PSCA Antibody Picoband®
SKU/Catalog Number
PB9351
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PSCA Antibody Picoband® catalog # PB9351. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PSCA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9351)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9351 is reactive to PSCA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
25 kDa
Calculated molecular weight
12912 MW
Background of PSCA
PSCA is also known as PRO232. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene includes a polymorphism that results in an upstream start codon in some individuals; this polymorphism is thought to be associated with a risk for certain gastric and bladder cancers. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9351 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Positive Control
WB: Rat Stomach Tissue, Mouse Stomach Tissue
Validation Images & Assay Conditions
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Anti-PSCA Picoband antibody, PB9351, Western blotting
All lanes: Anti PSCA (PB9351) at 0.5ug/ml
Lane 1: Rat Stomach Tissue Lysate at 50ug
Lane 2: Mouse Stomach Tissue Lysate at 50ug
Predicted bind size: 13KD
Observed bind size: 25KD
Protein Target Info & Infographic
Gene/Protein Information For PSCA (Source: Uniprot.org, NCBI)
Gene Name
PSCA
Full Name
Prostate stem cell antigen
Weight
12912 MW
Alternative Names
Prostate stem cell antigen;PSCA;UNQ206/PRO232; PSCA PRO232 prostate stem cell prostate stem cell
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PSCA, check out the PSCA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PSCA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PSCA Antibody Picoband® (PB9351)
Hello CJ!
PB9351 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
PSCA rs2294008 polymorphism contributes to the decreased risk for cervical cancer in a Chinese population
Zhao Z, Ma W, Zeng G, Qi D, Ou L, Liang Y. Urol Oncol. 2013 Apr;31(3):343-51. Doi: 10.1016/J.Urolonc.2011.02.004. Epub 2011 Mar 23. Small Interference Rna-Mediated Silencing Of Prostate Stem Cell Antigen Attenuates Growth, Reduces Migration And In...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PSCA Antibody Picoband®
Question
My question regarding product PB9351, anti-PSCA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9351 anti-PSCA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-08
Question
We are currently using anti-PSCA antibody PB9351 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
The anti-PSCA antibody (PB9351) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-PSCA antibody for WB for rat prostatic carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat prostatic carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-07-22
Answer
It shows on the product datasheet, PB9351 anti-PSCA antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat prostatic carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-22
Question
My colleagues were satisfied with the WB result of your anti-PSCA antibody. However we have seen positive staining in melanoma cell membrane using this antibody. Is that expected? Could you tell me where is PSCA supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-17
Answer
According to literature, melanoma does express PSCA. Generally PSCA expresses in cell membrane. Regarding which tissues have PSCA expression, here are a few articles citing expression in various tissues:
Melanoma, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 9465086
Urothelium, Pubmed ID: 10973799
Boster Scientific Support
Answered: 2019-05-17
Question
See attached the WB image, lot number and protocol we used for prostatic carcinoma using anti-PSCA antibody PB9351. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-09
Question
Is this PB9351 anti-PSCA antibody reactive to the isotypes of PSCA?
J. Kulkarni
Verified customer
Asked: 2018-01-12
Answer
The immunogen of PB9351 anti-PSCA antibody is E.coli-derived human PSCA recombinant protein (Position: L21-S95). Human PSCA shares 66% amino acid (aa) sequence identity with mouse PSCA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for prostatic carcinoma using anti-PSCA antibody PB9351. Let me know if you need anything else.
A. Banerjee
Verified customer
Asked: 2017-11-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-17
Question
Will anti-PSCA antibody PB9351 work for WB with prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2017-08-23
Answer
According to the expression profile of prostatic carcinoma, PSCA is highly expressed in prostatic carcinoma. So, it is likely that anti-PSCA antibody PB9351 will work for WB with prostatic carcinoma.
Boster Scientific Support
Answered: 2017-08-23
Question
I was wanting to use to test anti-PSCA antibody PB9351 on rat prostatic carcinoma for research purposes, then I may be interested in using anti-PSCA antibody PB9351 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Banerjee
Verified customer
Asked: 2016-12-06
Answer
The products we sell, including anti-PSCA antibody PB9351, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2016-12-06
Question
I see that the anti-PSCA antibody PB9351 works with WB, what is the protocol used to produce the result images on the product page?
C. Bhatt
Verified customer
Asked: 2016-08-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-08-08
Question
We have seen staining in human urothelium. Do you have any suggestions? Is anti-PSCA antibody supposed to stain urothelium positively?
A. Patel
Verified customer
Asked: 2015-11-27
Answer
From literature urothelium does express PSCA. From Uniprot.org, PSCA is expressed in fundus of stomach, prostatic carcinoma, urothelium, melanoma, among other tissues. Regarding which tissues have PSCA expression, here are a few articles citing expression in various tissues:
Melanoma, Pubmed ID: 15489334
Prostatic carcinoma, Pubmed ID: 9465086
Urothelium, Pubmed ID: 10973799
Boster Scientific Support
Answered: 2015-11-27
Question
Do you have a BSA free version of anti-PSCA antibody PB9351 available?
K. Taylor
Verified customer
Asked: 2015-09-30
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-PSCA antibody PB9351 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-30
Question
Will PB9351 anti-PSCA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Wu
Verified customer
Asked: 2014-05-26
Answer
As indicated on the product datasheet, PB9351 anti-PSCA antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-05-26
Question
We want using your anti-PSCA antibody for regulation of neurotransmitter receptor activity studies. Has this antibody been tested with western blotting on rat stomach tissue? We would like to see some validation images before ordering.
L. Parker
Verified customer
Asked: 2014-01-31
Answer
We appreciate your inquiry. This PB9351 anti-PSCA antibody is validated on rat stomach tissue, tissue lysate, mouse stomach tissue lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-01-31
Question
Is a blocking peptide available for product anti-PSCA antibody (PB9351)?
P. Krishna
Verified customer
Asked: 2013-11-27
Answer
We do provide the blocking peptide for product anti-PSCA antibody (PB9351). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-11-27
