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Product Info Summary
| SKU: | PA1386 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-HSD17B6 Antibody Picoband®
SKU/Catalog Number
PA1386
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HSD17B6 Antibody catalog # PA1386. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSD17B6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1386)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human HSD17B6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1386 is reactive to HSD17B6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
35966 MW
Background of HSD17B6
Hydroxysteroid 17-beta dehydrogenase 6 is an enzyme that in humans is encoded by the HSD17B6 gene. The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. Baker ME et al point out expression of 17beta-HSDs had an important role in the early evolution of the physiological response to androgens and estrogens. Biswas and Russell concluded that 17beta-HSD6 and RoDH play opposing roles in androgen action; 17beta-HSD6 inactivates 3alpha-adiol by conversion to androsterone and RoDH activates 3alpha-adiol by conversion to dihydrotestosterone. The synthesis of an active steroid hormone by back conversion of an inactive metabolite represents a potentially important mechanism by which the steady state level of a transcriptional effector can be regulated.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1386 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue, MCF-7 Whole Cell
IHC: human liver cancer tissue
Validation Images & Assay Conditions
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Anti-HSD17B6 antibody, PA1386, Western blotting
All lanes: Anti HSD17B6 (PA1386) at 0.5ug/ml
Lane 1: Human Placenta Tissue Lysate at 50ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
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Figure 2. IHC analysis of HSD17B6 using anti-HSD17B6 antibody (PA1386).
HSD17B6 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-HSD17B6 Antibody (PA1386) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For HSD17B6 (Source: Uniprot.org, NCBI)
Gene Name
HSD17B6
Full Name
17-beta-hydroxysteroid dehydrogenase type 6
Weight
35966 MW
Superfamily
short-chain dehydrogenases/reductases (SDR) family
Alternative Names
17-beta-hydroxysteroid dehydrogenase type 6;17-beta-HSD 6;17-beta-HSD6;1.1.1.105;1.1.1.239;1.1.1.62;3-alpha->beta-hydroxysteroid epimerase;3-alpha->beta-HSE;Oxidative 3-alpha hydroxysteroid dehydrogenase;Short chain dehydrogenase/reductase family 9C member 6;HSD17B6;RODH, SDR9C6; HSD17B6 HSE, RODH, SDR9C6 hydroxysteroid 17-beta dehydrogenase 6 17-beta-hydroxysteroid dehydrogenase type 6|17-beta-HSD 6|17-beta-hydroxysteroid dehydrogenase type 6 variant 1|17-beta-hydroxysteroid dehydrogenase type 6 variant 2|17-beta-hydroxysteroid dehydrogenase type 6 variant 3|3(alpha->beta)-hydroxysteroid epimerase|3(alpha->beta)-hydroxysteroid epimerasel|3-alpha->beta-HSE|3-hydroxysteroid epimerase|NAD+ -dependent 3 alpha-hydroxysteroid dehydrogenase 3-hydroxysteroid epimerase|hydroxysteroid (17-beta) dehydrogenase 6 homolog|oxidative 3-alpha-hydroxysteroid-dehydrogenase|oxidoreductase|retinol dehydrogenase|short chain dehydrogenase/reductase family 9C member 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSD17B6, check out the HSD17B6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSD17B6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSD17B6 Antibody Picoband® (PA1386)
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Customer Q&As
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4 Customer Q&As for Anti-HSD17B6 Antibody Picoband®
Question
Is there a BSA free version of anti-HSD17B6 antibody PA1386 available?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HSD17B6 antibody PA1386 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-16
Question
I was wanting to use your anti-HSD17B6 antibody for WB for human prostate on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human prostate identification?
Verified Customer
Verified customer
Asked: 2019-01-31
Answer
It shows on the product datasheet, PA1386 anti-HSD17B6 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human prostate in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-01-31
Question
We are currently using anti-HSD17B6 antibody PA1386 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
F. Collins
Verified customer
Asked: 2015-04-23
Answer
The anti-HSD17B6 antibody (PA1386) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-23
Question
Our lab want to know about to test anti-HSD17B6 antibody PA1386 on human prostate for research purposes, then I may be interested in using anti-HSD17B6 antibody PA1386 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Mitchell
Verified customer
Asked: 2014-05-15
Answer
The products we sell, including anti-HSD17B6 antibody PA1386, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-05-15
