Anti-PRSS27 Antibody Picoband™

Product Name

Anti-PRSS27 Antibody Picoband™

View all Marapsin/Pancreasin Antibodies

SKU/Catalog Number

A14210-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PRSS27 Antibody Picoband™ catalog # A14210-1. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PRSS27 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14210-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PRSS27 recombinant protein (Position: A23-K290).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14210-1 is reactive to PRSS27 in Human, Mouse, Rat

Applications

A14210-1 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

32 kDa

Calculated molecular weight

64099 MW

Background of Marapsin/Pancreasin

This gene is located within a large protease gene cluster on chromosome 16. It belongs to the group-1 subfamily of serine proteases. The encoded protein is a secreted tryptic serine protease and is expressed mainly in the pancreas. Alternative splicing results in multiple transcript variants.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of PRSS27 using anti-PRSS27 antibody (A14210-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat stomach tissue lysates,
Lane 2: mouse pancreas tissue lysates,
Lane 3: mouse MFC whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRSS27 antigen affinity purified polyclonal antibody (Catalog # A14210-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRSS27 at approximately 32 kDa. The expected band size for PRSS27 is at 28,32 kDa.

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Figure 2. Flow Cytometry analysis of JK cells using anti-PRSS27 antibody (A14210-1).
Overlay histogram showing JK cells stained with A14210-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-PRSS27 Antibody (A14210-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For PRSS27 (Source: Uniprot.org, NCBI)

Alternative Names

CAP-2; CAPH2; channel-activating protease 2; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.4; EC 3.4.21.43; Marapsin; MPN; MPNMarapsin; Pancreasin; protease, serine 27; PRSS27; serine protease 27 PRSS27 CAPH2, MPN serine protease 27 serine protease 27|channel-activating protease 2|marapsin|pancreasin|protease, serine 27

For more info on PRSS27, check out the PRSS27 Infographic

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In this infographic, you will see the following information for PRSS27: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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