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Product Info Summary
| SKU: | RP1067 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-SFRP4 Antibody Picoband®
SKU/Catalog Number
RP1067
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-SFRP4 Antibody catalog # RP1067. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFRP4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1067)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SFRP4 recombinant protein (Position: E140-V346). Human SFRP4 shares 90% and 90.3% amino acid (aa) sequence identity with mouse and rat SFRP4, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1067 is reactive to SFRP4 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
39827 MW
Background of sFRP-4
Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. It is mappaead to 7p14.1. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. And SFRP4 is a hub gene in a Type 2 Diabetes-associated gene coexpression module in human islets, and reduces glucose-induced insulin secretion through decreased β-cell exocytosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1067 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: Rat Cardiac Muscle Tissue, SKOV Whole Cell
Validation Images & Assay Conditions
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Anti-SFRP4 Picoband antibody, RP1067, Western blotting
All lanes: Anti SFRP4 (RP1067) at 0.5ug/ml
Lane 1: Rat Cardiac Muscle Tissue Lysate at 50ug
Lane 2: SKOV Whole Cell Lysate at 40ug
Predicted bind size: 40KD
Observed bind size: 40KD
Protein Target Info & Infographic
Gene/Protein Information For SFRP4 (Source: Uniprot.org, NCBI)
Gene Name
SFRP4
Full Name
Secreted frizzled-related protein 4
Weight
39827 MW
Superfamily
secreted frizzled-related protein (sFRP) family
Alternative Names
Secreted frizzled-related protein 4;sFRP-4;Frizzled protein, human endometrium;FrpHE;SFRP4;FRPHE; SFRP4 FRP-4, FRPHE, FRZB-2, PYL, sFRP-4 secreted frizzled related protein 4 secreted frizzled-related protein 4|frizzled protein, human endometrium|secreted frizzled-related protein 4; secreted frizzled-related protein 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SFRP4, check out the SFRP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SFRP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFRP4 Antibody Picoband® (RP1067)
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No publications found for RP1067
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SFRP4 Antibody Picoband®
Question
Would anti-SFRP4 antibody RP1067 work on primate WB with brain?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
Our lab technicians have not tested anti-SFRP4 antibody RP1067 on primate. You can run a BLAST between primate and the immunogen sequence of anti-SFRP4 antibody RP1067 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-14
Question
We are currently using anti-SFRP4 antibody RP1067 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, rat. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-19
Answer
The anti-SFRP4 antibody (RP1067) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-19
Question
See attached the WB image, lot number and protocol we used for ovary using anti-SFRP4 antibody RP1067. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-09
Question
Do you have a BSA free version of anti-SFRP4 antibody RP1067 available?
D. Krishna
Verified customer
Asked: 2016-12-12
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-SFRP4 antibody RP1067 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-12-12
Question
I was wanting to use your anti-SFRP4 antibody for WB for rat ovary on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat ovary identification?
R. Collins
Verified customer
Asked: 2014-12-18
Answer
It shows on the product datasheet, RP1067 anti-SFRP4 antibody has been tested for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-18
Question
My lab would like to test anti-SFRP4 antibody RP1067 on rat ovary for research purposes, then I may be interested in using anti-SFRP4 antibody RP1067 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Moore
Verified customer
Asked: 2013-02-15
Answer
The products we sell, including anti-SFRP4 antibody RP1067, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-02-15
