Product Info Summary
SKU: | A00601-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Prolactin/PRL Antibody Picoband™
SKU/Catalog Number
A00601-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-Prolactin/PRL Antibody Picoband™ catalog # A00601-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Prolactin/PRL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00601-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Prolactin/PRL recombinant protein (Position: A36-D206).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00601-1 is reactive to PRL in Human, Mouse, Rat
Applications
A00601-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
26 kDa
Calculated molecular weight
Background of Prolactin
Prolactin (PRL) also known as luteotropic hormone (LTH) is a protein that in humans is encoded by the PRL gene. Prolactin is a peptide hormone discovered by Henry Friesen. Although it is perhaps best known for its role in lactation, prolactin already existed in the oldest known vertebrates—fishes—where its most important functions were probably related to control of water and salt balance. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Prolactin/PRL using anti-Prolactin/PRL antibody (A00601-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prolactin/PRL antigen affinity purified polyclonal antibody (Catalog # A00601-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prolactin/PRL at approximately 26 kDa. The expected band size for Prolactin/PRL is at 26 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRL (Source: Uniprot.org, NCBI)
Gene Name
PRL
Full Name
Prolactin
Weight
Superfamily
somatotropin/prolactin family
Alternative Names
PRL; Prolactin PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRL, check out the PRL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prolactin/PRL Antibody Picoband™ (A00601-1)
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