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Product Info Summary
| SKU: | EK0593 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, citrate). |
Product info
Product Name
Human Prolactin ELISA Kit PicoKine®
SKU/Catalog Number
EK0593
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Human PRL in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Prolactin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0593)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: L29-C227
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0593 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0593 is reactive to PRL in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, citrate).
Application Guarantee
EK0593 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Prolactin
Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis,angiogenesis and is involved in the regulation of blood clotting through several pathways.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0593-CAP | Anti-Human PRL Pre-coated 96-well strip microplate | 1 |
| EK0593-ST | Human PRL Standard | 2 vials, 10 ng/tube |
| EK0593-DA | Human PRL Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.027 | 0.123 | 0.217 | 0.409 | 0.780 | 1.237 | 1.553 | 1.933 |
Data Example Images
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Human Prolactin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Prolactin, Dilution ratio of 1:10, concentration in serum and plasma is 2-127 ng/ml..
Some articles we found to cite concentrations of Prolactin in samples: 15083880 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Prolactin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 278 | 1989 | 4706 | 274 | 1928 | 5020 |
| Standard deviation | 18.07 | 95.47 | 338.83 | 21.64 | 129.17 | 371.48 |
| CV (%) | 6.5% | 4.8% | 7.2% | 7.9% | 6.7% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Prolactin in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 278 | 266 | 323 | 299 | 291 | 21.68 | 7.4% |
| Sample 2 | 1989 | 1892 | 1944 | 1862 | 1921 | 48.66 | 2.5% |
| Sample 3 | 4706 | 4695 | 5058 | 4926 | 4846 | 153.08 | 3.1% |
Protein Target Info & Infographic
Gene/Protein Information For PRL (Source: Uniprot.Org, NCBI)
Gene Name
PRL
Full Name
Prolactin
Weight
25876 MW
Superfamily
somatotropin/prolactin family
Alternative Names
Prolactin;PRL;PRL; PRL GHA1 prolactin prolactin|decidual prolactin|growth hormone A1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PRL, check out the PRL Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PRL: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human Prolactin ELISA Kit PicoKine® (EK0593)
Hello CJ!
EK0593 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chronic endometritis modifies decidualization in human endometrial stromal cells
Chronic endometritis modifies decidualization in human endometrial stromal cells
Garrido-Gomez T, Dominguez F, Qui?onero A, Diaz-Gimeno P, Kapidzic M, Gormley M, Ona K, Padilla-Iserte P, McMaster M, Genbacev O, Perales A, Fisher SJ, Sim
Xia Y, Qi F, Zou J, Yao Z. Brain Res. 2014 Dec 10;1592:34-43. Influenza A(H1N1) Vaccination During Early Pregnancy Transiently Promotes Hippocampal Neurogenesis And Working Memory. Involvement Of Th1/Th2 Balance.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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6 Customer Q&As for Human Prolactin ELISA Kit PicoKine®
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Prolactin in Human plasma? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-05-30
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Prolactin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
T. Dayal
Verified customer
Asked: 2019-12-08
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-08
Question
Q: how to proceed with the analysis of ELISA data? I have performed Prolactin level in plasma.
W. Campbell
Verified customer
Asked: 2019-11-25
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-25
Question
Q: Can Prolactin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
K. Babu
Verified customer
Asked: 2019-06-15
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-06-15
Question
Can EK0593 detect rhesus monkey prolactin?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
We have not used EK0593 with Rhesus Monkey samples. The sequence homology with rhesus monkey is 98%. https://www.uniprot.org/blast/uniprot/B201812033707B37BB34871DC742D8A439176A1E10305B6U We are uncertain if the kit will detect rhesus monkey prolactin. So,we suggest that a pilot test is ran with EK0593.
Boster Scientific Support
Answered: 2018-08-09
Question
Q: is it okay to use EDTA plasma as samples in Human Prolactin Picokine® ELISA Kit (Catalog # EK0593)?
W. Krishna
Verified customer
Asked: 2017-01-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Prolactin causing disruption of its protein structure. Prolactin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Prolactin ELISA, treating samples with various anticoagulants and decided that heparin or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-01-26
