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Product Info Summary
| SKU: | PA1026 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Connexin 43/GJA1 Antibody Picoband®
View all Connexin 43/GJA1 Antibodies
SKU/Catalog Number
PA1026
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Connexin 43/GJA1 Antibody catalog # PA1026. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Connexin 43/GJA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1026)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1026 is reactive to GJA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
43008 MW
Background of Connexin 43/GJA1
Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1 (GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene, which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1026 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence, 2μg/ml, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: human placenta tissue, rat brain tissue, rat heart tissue, mouse brain tissue, mouse heart tissue
IHC: rat cardiac muscle tissue
IF: rat heart tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat heart tissue lysates,
Lane 4: mouse brain tissue lysates,
Lane 5: mouse heart tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 43/GJA1 antigen affinity purified polyclonal antibody (Catalog # PA1026) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 43/GJA1 at approximately 43KD. The expected band size for Connexin 43/GJA1 is at 43KD.
Click image to see more details
Figure 2. IHC analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026).
Connexin 43/GJA1 was detected in paraffin-embedded section of rat cardiac muscle tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Connexin 43/GJA1 Antibody (PA1026) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IF analysis of Connexin 43/GJA1 using anti-Connexin 43/GJA1 antibody (PA1026).
Connexin 43/GJA1 was detected in paraffin-embedded section of rat heart tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Connexin 43/GJA1 Antibody (PA1026) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C.. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GJA1 (Source: Uniprot.org, NCBI)
Gene Name
GJA1
Full Name
Gap junction alpha-1 protein
Weight
43008 MW
Superfamily
connexin family
Alternative Names
Gap junction alpha-1 protein;Connexin-43;Cx43;Gap junction 43 kDa heart protein;GJA1;GJAL; GJA1 AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA gap junction protein alpha 1 gap junction alpha-1 protein|connexin-43|gap junction 43 kDa heart protein|gap junction protein, alpha 1, 43kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJA1, check out the GJA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 43/GJA1 Antibody Picoband® (PA1026)
Hello CJ!
PA1026 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Y,Bai H,Guo F,Thai PN,Luo X,Zhang P,Yang C,Feng X,Zhu D,Guo J,Liang P,Xu Z,Yang H,Lu X.PGC-1α activator ZLN005 promotes maturation of cardiomyocytes derived from human embryonic stem cells.Aging (Albany NY).2020 Apr 28;12(8):7411-7430.doi:10.18632/agi
Species: Human
PA1026 usage in article: APP:WB, SAMPLE:CARDIOMYOCYTES, DILUTION:1:400
Effect of celecoxib on protein expression of FAK and Cx43 in DMBA induced rat tongue carcinoma cells B-Z Shan 1, B Guo, Y-S Li, X-F Sun Eur Rev Med Pharmacol Sci. 2019 Nov;23(21):9454-9463. doi: 10.26355/eurrev_201911_19439.
Ectoderm mesenchymal stem cells promote differentiation and maturation of oligodendrocyte precursor cells
Effect of Novel Gasotransmitter hydrogen sulfide on renal fibrosis and connexins expression in diabetic rats
miR-381suppresses C/EBP?-dependent Cx43 expression in breast cancer cells
Identification of miR-200a as a novel suppressor of connexin 43in breast cancer cells
Argirein alleviates stress-induced and diabetic hypogonadism in rats via normalizing testis endothelin receptor A and connexin 43
CPU86017-RS attenuate hypoxia-induced testicular dysfunction in mice by normalizing androgen biosynthesis genes and pro-inflammatory cytokines
Engineered myocardial tissues constructed in vivo using cardiomyocyte-like cells derived from bone marrow mesenchymal stem cells in rats
Li H, Tang M, Liang H, Li Y, Wang J, Song Y, Zheng Y, Xi J, Zhang J, Hescheler J, Zhu M. Cell Physiol Biochem. 2013;32(1):53-63. Doi: 10.1159/000350124. Epub 2013 Jul 4. Coculture Of Embryonic Ventricular Myocytes And Mouse Embryonic Stem Cell Enh...
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Customer Q&As
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16 Customer Q&As for Anti-Connexin 43/GJA1 Antibody Picoband®
Question
Our lab used your anti-Connexin 43/GJA1 antibody for WB on heart muscle in a previous project. I am using mouse, and I plan to use the antibody for IHC-P next. I am looking for examining heart muscle as well as cerebellum in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-04-08
Answer
I looked at the website and datasheets of our anti-Connexin 43/GJA1 antibody and it appears that PA1026 has been validated on mouse in both WB and IHC-P. Thus PA1026 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-08
Question
I see that the anti-Connexin 43/GJA1 antibody PA1026 works with IHC-P, what is the protocol used to produce the result images on the product page?
D. Anderson
Verified customer
Asked: 2020-02-06
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-06
Question
Please see the WB image, lot number and protocol we used for pigmented layer of retina using anti-Connexin 43/GJA1 antibody PA1026. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-27
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pigmented layer of retina using anti-Connexin 43/GJA1 antibody PA1026. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-08
Question
Does PA1026 anti-Connexin 43/GJA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-10-09
Answer
You can see on the product datasheet, PA1026 anti-Connexin 43/GJA1 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-09
Question
Do you have a BSA free version of anti-Connexin 43/GJA1 antibody PA1026 available?
Verified Customer
Verified customer
Asked: 2019-09-06
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Connexin 43/GJA1 antibody PA1026 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-06
Question
Is this PA1026 anti-Connexin 43/GJA1 antibody reactive to the isotypes of GJA1?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
The immunogen of PA1026 anti-Connexin 43/GJA1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 43(366-382aa RASSRASSRPRPDDLEI), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-01
Question
We need to test anti-Connexin 43/GJA1 antibody PA1026 on rat pigmented layer of retina for research purposes, then I may be interested in using anti-Connexin 43/GJA1 antibody PA1026 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
The products we sell, including anti-Connexin 43/GJA1 antibody PA1026, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-07-08
Question
I have a question about product PA1026, anti-Connexin 43/GJA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1026 anti-Connexin 43/GJA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-09
Question
you antibody using your anti-Connexin 43/GJA1 antibody for regulation of transmembrane transporter activity studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-16
Answer
Thank you for your inquiry. This PA1026 anti-Connexin 43/GJA1 antibody is tested on human placenta tissue, rat heart tissue, brain tissue, mouse heart tissue. It is guaranteed to work for IF, IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-16
Question
I was wanting to use your anti-Connexin 43/GJA1 antibody for IHC-P for rat pigmented layer of retina on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat pigmented layer of retina identification?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
It shows on the product datasheet, PA1026 anti-Connexin 43/GJA1 antibody has been validated for IF, IHC-P, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat pigmented layer of retina in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-25
Question
Is a blocking peptide available for product anti-Connexin 43/GJA1 antibody (PA1026)?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We do provide the blocking peptide for product anti-Connexin 43/GJA1 antibody (PA1026). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-10-11
Question
We were content with the WB result of your anti-Connexin 43/GJA1 antibody. However we have seen positive staining in heart muscle cell membrane using this antibody. Is that expected? Could you tell me where is GJA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-24
Answer
Based on literature, heart muscle does express GJA1. Generally GJA1 expresses in cell membrane. Regarding which tissues have GJA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Heart muscle, Pubmed ID: 1696265
Boster Scientific Support
Answered: 2017-07-24
Question
We have observed staining in human brain. Do you have any suggestions? Is anti-Connexin 43/GJA1 antibody supposed to stain brain positively?
H. Thomas
Verified customer
Asked: 2015-12-17
Answer
From what I have seen in literature brain does express GJA1. From what I have seen in Uniprot.org, GJA1 is expressed in pigmented layer of retina, heart muscle, cerebellum, brain, among other tissues. Regarding which tissues have GJA1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebellum, Pubmed ID: 14702039
Heart muscle, Pubmed ID: 1696265
Boster Scientific Support
Answered: 2015-12-17
Question
Would anti-Connexin 43/GJA1 antibody PA1026 work for IHC-P with pigmented layer of retina?
A. Gonzalez
Verified customer
Asked: 2014-05-02
Answer
According to the expression profile of pigmented layer of retina, GJA1 is highly expressed in pigmented layer of retina. So, it is likely that anti-Connexin 43/GJA1 antibody PA1026 will work for IHC-P with pigmented layer of retina.
Boster Scientific Support
Answered: 2014-05-02
Question
We are currently using anti-Connexin 43/GJA1 antibody PA1026 for human tissue, and we are content with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
G. Kulkarni
Verified customer
Asked: 2013-10-29
Answer
The anti-Connexin 43/GJA1 antibody (PA1026) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-10-29
