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Key=description, value=The Connexin 43 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Connexin 43 protein expression profile in cells. The kit can be used for measuring the relative amounts of Connexin 43 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Connexin 43.
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Key=subcellular_localization, value=Cell membrane; Multi-pass membrane protein. Cell junction, gap junction. Endoplasmic reticulum. Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.
Key=tissue_specificity, value=Expressed in the heart and fetal cochlea.
Key=protein_function, value= Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).
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Connexin 43 Colorimetric Cell-Based ELISA Kit

Connexin 43/GJA1 ELISA kit, cell-based

The Connexin 43 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Connexin 43 protein expression profile in cells. The kit can be used for measuring the relative amounts of Connexin 43 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Connexin 43.

Product Info Summary

SKU: EKC1134
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA
Sample Types: Cell lines

Product Name

Connexin 43 Colorimetric Cell-Based ELISA Kit

See all Connexin 43/GJA1 products

SKU/Catalog Number

EKC1134

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The Connexin 43 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Connexin 43 protein expression profile in cells. The kit can be used for measuring the relative amounts of Connexin 43 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Connexin 43.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

Connexin 43 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1134)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1134 is reactive to GJA1 in Human, Mouse, Rat samples

Validated Sample Types

Cell lines

Application Guarantee

EKC1134 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-Connexin 43 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

Gene/Protein Information For GJA1 (Source: Uniprot.Org, NCBI)

Gene Name

GJA1

Full Name

Gap junction alpha-1 protein

Weight

43008 MW

Superfamily

connexin family

Alternative Names

Connexin 43;CX43;CXA1;CXN-43;Gap junction 43 kDa heart protein;Gap junction alpha-1 protein;GJA1 GJA1 AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA gap junction protein alpha 1 gap junction alpha-1 protein|connexin-43|gap junction 43 kDa heart protein|gap junction protein, alpha 1, 43kDa

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GJA1, check out the GJA1 Infographic

GJA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GJA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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