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Product Info Summary
| SKU: | M02447 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Prion Protein PRNP Rabbit Monoclonal Antibody
View all Prion protein Antibodies
SKU/Catalog Number
M02447
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Prion Protein PRNP Rabbit Monoclonal Antibody catalog # M02447. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Prion Protein PRNP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02447)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
EHH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Prion Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02447 is reactive to PRNP in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
27661 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02447 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: Hunam fetal brain, rat brain tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRNP using anti-PRNP antibody (M02447).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP antigen affinity purified monoclonal antibody (Catalog # M02447) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRNP at approximately 30 kDa. The expected band size for PRNP is at 28 kDa.
Click image to see more details
Western blot analysis of Prion Protein expression in Hunam fetal brain lysate.
Protein Target Info & Infographic
Gene/Protein Information For PRNP (Source: Uniprot.org, NCBI)
Gene Name
PRNP
Full Name
Alternative prion protein
Weight
27661 MW
Alternative Names
Major prion protein;PrP;ASCR;PrP27-30;PrP33-35C;CD230;PRNP;ALTPRP, PRIP, PRP; PRNP ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30 prion protein major prion protein|alternative prion protein|CD230 |prion-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRNP, check out the PRNP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRNP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Prion Protein PRNP Rabbit Monoclonal Antibody (M02447)
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No publications found for M02447
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Prion Protein PRNP Rabbit Monoclonal Antibody
Question
What is the concentration for M02447?
Verified customer
Asked: 2021-02-08
Answer
The concentration for the Anti-Prion Protein PRNP Rabbit Monoclonal Antibody (M02447) is 1mg/ml.
Boster Scientific Support
Answered: 2021-02-15
Question
I would like to test anti-Prion Protein Rabbit Monoclonal antibody M02447 on rat brain for research purposes, then I may be interested in using anti-Prion Protein Rabbit Monoclonal antibody M02447 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The products we sell, including anti-Prion Protein Rabbit Monoclonal antibody M02447, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-04-29
Question
We have been able to see staining in rat brain. Do you have any suggestions? Is anti-Prion Protein Rabbit Monoclonal antibody supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-04-17
Answer
According to literature brain does express PRNP. According to Uniprot.org, PRNP is expressed in brodmann (1909) area 23, brain, blood, prostate, brain ovary, leukemic t-cell, among other tissues. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2020-04-17
Question
We bought anti-Prion Protein Rabbit Monoclonal antibody for Flow Cytometry on blood in a previous project. I am using human, and We want to use the antibody for IHC next. I am interested in examining blood as well as brodmann (1909) area 23 in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
I have checked the website and datasheets of our anti-Prion Protein Rabbit Monoclonal antibody and I see that M02447 has been tested on human in both Flow Cytometry and IHC. Thus M02447 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-14
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Prion Protein Rabbit Monoclonal antibody M02447. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-04
Question
Would anti-Prion Protein Rabbit Monoclonal antibody M02447 work for IF with brain?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
According to the expression profile of brain, PRNP is highly expressed in brain. So, it is likely that anti-Prion Protein Rabbit Monoclonal antibody M02447 will work for IF with brain.
Boster Scientific Support
Answered: 2020-01-09
Question
Is a blocking peptide available for product anti-Prion Protein Rabbit Monoclonal antibody (M02447)?
K. Parker
Verified customer
Asked: 2019-11-20
Answer
We do provide the blocking peptide for product anti-Prion Protein Rabbit Monoclonal antibody (M02447). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-20
Question
I was wanting to use your anti-Prion Protein Rabbit Monoclonal antibody for IF for rat brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat brain identification?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
It shows on the product datasheet, M02447 anti-Prion Protein Rabbit Monoclonal antibody has been validated for Flow Cytometry, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-07
Question
Do you have a BSA free version of anti-Prion Protein Rabbit Monoclonal antibody M02447 available?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Prion Protein Rabbit Monoclonal antibody M02447 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-02
Question
My question regarding product M02447, anti-Prion Protein Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02447 anti-Prion Protein Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-18
Question
I see that the anti-Prion Protein Rabbit Monoclonal antibody M02447 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-03
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-06-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Prion Protein Rabbit Monoclonal antibody M02447. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Does M02447 anti-Prion Protein Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-01-01
Answer
As indicated on the product datasheet, M02447 anti-Prion Protein Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-01
Question
We are currently using anti-Prion Protein Rabbit Monoclonal antibody M02447 for rat tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
H. Krishna
Verified customer
Asked: 2017-08-08
Answer
The anti-Prion Protein Rabbit Monoclonal antibody (M02447) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-08-08
Question
Is this M02447 anti-Prion Protein Rabbit Monoclonal antibody reactive to the isotypes of PRNP?
E. Banerjee
Verified customer
Asked: 2015-02-25
Answer
The immunogen of M02447 anti-Prion Protein Rabbit Monoclonal antibody is A synthesized peptide derived from human Prion Protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-25
Question
Our lab were well pleased with the WB result of your anti-Prion Protein Rabbit Monoclonal antibody. However we have been able to see positive staining in leukemic t-cell cell membrane using this antibody. Is that expected? Could you tell me where is PRNP supposed to be expressed?
A. Krishna
Verified customer
Asked: 2013-12-25
Answer
Based on literature, leukemic t-cell does express PRNP. Generally PRNP expresses in cell membrane. Regarding which tissues have PRNP expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 1363802, 1678248
Brain, and Ovary, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19349973
Prostate, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2013-12-25
