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Product Info Summary
| SKU: | A07687-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CINP Antibody Picoband®
SKU/Catalog Number
A07687-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CINP Antibody Picoband® catalog # A07687-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CINP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07687-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse CINP, which shares 85.7% and 100% amino acid (aa) sequence identity with human and rat CINP, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07687-1 is reactive to Cinp in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
32679 MW
Background of CINP
Cartilage intermediate layer protein 1 is a protein that in humans is encoded by the CILP gene. The protein encoded by this gene is reported to be a component of the DNA replication complex as well as a genome-maintenance protein. It may interact with proteins important for replication initiation and has been shown to bind chromatin at the G1 phase of the cell cycle and dissociate from chromatin with replication initiation. It may also serve to regulate checkpoint signaling as part of the DNA damage response. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07687-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Mouse
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Mouse
Positive Control
WB: mouse lung tissue, mouse RAW2647 whole cell
FCM: RAW2647 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CINP using anti-CINP antibody (A07687-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: mouse lung tissue lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CINP antigen affinity purified polyclonal antibody (Catalog # A07687-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CINP at approximately 26KD. The expected band size for CINP is at 26KD.
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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-CINP antibody (A07687-1).
Overlay histogram showing RAW264.7 cells stained with A07687-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CINP Antibody (A07687-1, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Cinp (Source: Uniprot.org, NCBI)
Gene Name
Cinp
Full Name
Cyclin-dependent kinase 2-interacting protein
Weight
32679 MW
Superfamily
CINP family
Alternative Names
Trem-like transcript 1 protein;TLT-1;Triggering receptor expressed on myeloid cells-like protein 1;TREML1;TLT1;UNQ1825/PRO3438; CINP cyclin dependent kinase 2 interacting protein cyclin-dependent kinase 2-interacting protein|CDK2-interacting protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cinp, check out the Cinp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cinp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CINP Antibody Picoband® (A07687-1)
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