Anti-PRDM16 Antibody

Product Name

Anti-PRDM16 Antibody

View all PRDM16/MEL1 Antibodies

SKU/Catalog Number

A00782

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-PRDM16 Antibody (Catalog # A00782). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.

Storage & Handling

PRDM16 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-PRDM16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00782)

Host

Rabbit

Contents

PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

PRDM16 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human PRDM16. The immunogen is located within amino acids 1120 - 1170 of PRDM16.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

LAMP-1 antibody is predicted to not cross-react with LAMP-2. Due to extensive post-translational modification, LAMP-1 often migrates at higher than predicted molecular weight in SDS-PAGE.

Reactive Species

A00782 is reactive to PRDM16 in Human, Mouse

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

140251 MW

Background of PRDM16/MEL1

PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00782 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

PRDM16 antibody can be used for detection of PRDM16 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For PRDM16 (Source: Uniprot.org, NCBI)

Alternative Names

MEL1, LVNC8, PFM13, CMD1LL, KIAA1675, MEL1, PR domain zinc finger protein 16, PR domain-containing protein 16 PRDM16 CMD1LL, KMT8F, LVNC8, MEL1, PFM13 PR/SET domain 16 histone-lysine N-methyltransferase PRDM16|MDS1/EVI1-like gene 1|PR domain 16|PR domain containing 16|PR domain zinc finger protein 16|transcription factor MEL1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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