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Product Info Summary
| SKU: | M00782 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-PRDM16 Antibody
View all PRDM16/MEL1 Antibodies
SKU/Catalog Number
M00782
Size
50 µl
Description
Boster Bio Anti-PRDM16 Antibody (Catalog # M00782). Tested in WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-PRDM16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00782)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1740CT793.52.44
Isotype
IgG1,k
Immunogen
This PRDM16 antibody is generated from a mouse immunized with a recombinant protein between 779-996 amino acids from PRDM16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00782 is reactive to PRDM16 in Human
Reconstitution
Calculated molecular weight
140251 Da
Background of PRDM16/MEL1
Binds DNA and functions as a transcriptional regulator. Functions in the differentiation of brown adipose tissue (BAT) which is specialized in dissipating chemical energy in the form of heat in response to cold or excess feeding while white adipose tissue (WAT) is specialized in the storage of excess energy and the control of systemic metabolism. Together with CEBPB, regulates the differentiation of myoblastic precursors into brown adipose cells. Functions also as a repressor of TGF-beta signaling. Isoform 4 may regulate granulocytes differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00782 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:4000
Validation Images & Assay Conditions
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All lanes : Anti-PRDM16 Antibody at 1:4000 dilution
Lane 1: Jurkat whole cell lysate
Lane 2: K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 140 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For PRDM16 (Source: Uniprot.org, NCBI)
Gene Name
PRDM16
Full Name
Histone-lysine N-methyltransferase PRDM16
Weight
140251 Da
Superfamily
PRDM16 family
Alternative Names
PR domain zinc finger protein 16, PR domain-containing protein 16, Transcription factor MEL1, MDS1/EVI1-like gene 1, PRDM16, KIAA1675, MEL1, PFM13 PRDM16 CMD1LL, KMT8F, LVNC8, MEL1, PFM13 PR/SET domain 16 histone-lysine N-methyltransferase PRDM16|MDS1/EVI1-like gene 1|PR domain 16|PR domain containing 16|PR domain zinc finger protein 16|transcription factor MEL1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRDM16, check out the PRDM16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRDM16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRDM16 Antibody (M00782)
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