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Product Info Summary
| SKU: | A01582-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Niemann Pick C2/NPC2 Antibody Picoband®
View all Niemann-Pick type C2 Antibodies
SKU/Catalog Number
A01582-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Niemann Pick C2/NPC2 Antibody Picoband® catalog # A01582-2. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Niemann Pick C2/NPC2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01582-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Niemann Pick C2, which shares 79.4% and 76.5% amino acid (aa) sequence identity with mouse and rat Niemann Pick C2, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01582-2 is reactive to NPC2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
16 kDa
Calculated molecular weight
16.57kDa
Background of Niemann-Pick type C2
NPC2 is a protein associated with Niemann-Pick disease, type C. This gene is mapped to chromosome 14q24.3. It encodes a protein containing a lipid recognition domain. The encoded protein may function in regulating the transport of cholesterol through the late endosomal/lysosomal system. Mutations in this gene have been associated with Niemann-Pick disease, type C2 and frontal lobe atrophy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01582-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot,0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section),0.5-1μg/ml
Positive Control
WB: human SK-OV-3 whole cell
IHC: human lung cancer tissue, human intestinal cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Niemann Pick C2 using anti-Niemann Pick C2 antibody (A01582-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human SK-OV-3 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Niemann Pick C2 antigen affinity purified polyclonal antibody (Catalog # A01582-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Niemann Pick C2 at approximately 16KD. The expected band size for Niemann Pick C2 is at 16KD.
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Figure 2. IHC analysis of Niemann Pick C2 using anti-Niemann Pick C2 antibody (A01582-2).
Niemann Pick C2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Niemann Pick C2 Antibody (A01582-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Niemann Pick C2 using anti-Niemann Pick C2 antibody (A01582-2).
Niemann Pick C2 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Niemann Pick C2 Antibody (A01582-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For NPC2 (Source: Uniprot.org, NCBI)
Gene Name
NPC2
Full Name
NPC intracellular cholesterol transporter 2
Weight
16.57kDa
Superfamily
NPC2 family
Alternative Names
Epididymal secretory protein E1; Human epididymis-specific protein 1; He1; Niemann-Pick disease type C2 protein; NPC2; HE1 NPC2 EDDM1, HE1 NPC intracellular cholesterol transporter 2 NPC intracellular cholesterol transporter 2|Niemann-Pick disease type C2 protein|epididymal protein 1|epididymis secretory sperm binding protein|human epididymis-specific protein 1|tissue-specific secretory protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NPC2, check out the NPC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NPC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Niemann Pick C2/NPC2 Antibody Picoband® (A01582-2)
Hello CJ!
A01582-2 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Identification of pimavanserin tartrate as a potent Ca2+-calcineurin-NFAT pathway inhibitor for glioblastoma therapy
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-Niemann Pick C2/NPC2 Antibody Picoband®
Question
Do you have a BSA free version of anti-Niemann Pick C2/NPC2 antibody A01582-2 available?
J. Wu
Verified customer
Asked: 2019-10-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Niemann Pick C2/NPC2 antibody A01582-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-17
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovary using anti-Niemann Pick C2/NPC2 antibody A01582-2. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-23
Question
We are currently using anti-Niemann Pick C2/NPC2 antibody A01582-2 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The anti-Niemann Pick C2/NPC2 antibody (A01582-2) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-26
Question
I was wanting to use your anti-Niemann Pick C2/NPC2 antibody for WB for human ovary on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human ovary identification?
Verified Customer
Verified customer
Asked: 2019-02-20
Answer
As indicated on the product datasheet, A01582-2 anti-Niemann Pick C2/NPC2 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human ovary in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-02-20
Question
Is a blocking peptide available for product anti-Niemann Pick C2/NPC2 antibody (A01582-2)?
Verified Customer
Verified customer
Asked: 2018-01-29
Answer
We do provide the blocking peptide for product anti-Niemann Pick C2/NPC2 antibody (A01582-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-01-29
Question
My question regarding product A01582-2, anti-Niemann Pick C2/NPC2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01582-2 anti-Niemann Pick C2/NPC2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-25
