Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband®

Product Name

Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband®

View all BMI-1 Antibodies

SKU/Catalog Number

PA1807

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Polycomb complex protein BMI-1 Bmi1 Antibody catalog # PA1807. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Polycomb complex protein BMI-1 Bmi1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1807)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Bmi1, different from the related mouse sequence by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1807 is reactive to BMI1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

36949 MW

Background of BMI-1

BMI1 (BMI1 polycomb ring finger oncogene),also known as RNF51, is a protein which in humans is encoded by the BMI1 gene. The Bmi1 gene is highly conserved in evolution as indicated by zoo blot hybridization with Bmi1 probes corresponding to the protein-encoding domain. By fluorescence in situ hybridization, the human BMI1 gene is assigned to chromosome 10p13. BMI1 has a key role in regulating the proliferative activity of normal stem and progenitor cells. Most importantly, they provided evidence that the proliferative potential of leukemic stem and progenitor cells lacking BMI1 is compromised because they eventually undergo proliferation arrest and show signs of differentiation and apoptosis, leading to transplant failure of the leukemia. Complementation studies showed that BMI1 completely rescues these proliferative defects. Deletion analysis showed that the RING finger and helix-turn-helix domains of BMI1 were required for life span extension and repression of the tumor suppressor p16 (INK4). BMI1 selectively extended the life span of these cultures. Confocal microscopy showed that BMI1 transiently colocalized with centromeres during interphase in HeLa cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1807 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: HELA Whole Cell, HT1080 Whole Cell, COLO320 Whole Cell, MCF-7 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For BMI1 (Source: Uniprot.org, NCBI)

Alternative Names

Polycomb complex protein BMI-1;Polycomb group RING finger protein 4;RING finger protein 51;BMI1;PCGF4, RNF51; BMI1 FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1 BMI1 proto-oncogene, polycomb ring finger polycomb complex protein BMI-1|B lymphoma Mo-MLV insertion region 1 homolog|BMI1 polycomb ring finger oncogene|BMI1 polycomb ring finger proto-oncogene|murine leukemia viral (bmi-1) oncogene homolog|polycomb group RING finger protein 4|polycomb group protein Bmi1|ring finger protein 51

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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