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Product Info Summary
| SKU: | A00160 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-PRC1 Antibody
SKU/Catalog Number
A00160
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-PRC1 Antibody (Catalog # A00160). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
PRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-PRC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00160)
Host
Rabbit
Contents
PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1. The immunogen is located within amino acids 350 - 400 of PRC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Reactive Species
A00160 is reactive to PRC1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
71607 MW
Background of PRC1
PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00160 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
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Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.
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Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For PRC1 (Source: Uniprot.org, NCBI)
Gene Name
PRC1
Full Name
Protein regulator of cytokinesis 1
Weight
71607 MW
Superfamily
MAP65/ASE1 family
Alternative Names
ASE1, Protein regulator of cytokinesis 1 PRC1 ASE1 protein regulator of cytokinesis 1 protein regulator of cytokinesis 1|anaphase spindle elongation 1 homolog|protein regulating cytokinesis 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRC1, check out the PRC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRC1 Antibody (A00160)
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