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Product Info Summary
| SKU: | PB9814 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PRC1 Antibody Picoband®
SKU/Catalog Number
PB9814
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-PRC1 Antibody Picoband® catalog # PB9814. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PRC1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9814)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PRC1 recombinant protein (Position: M1-E233). Human PRC1 shares 85% amino acid (aa) sequence identity with mouse PRC1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9814 is reactive to PRC1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa, 80 kDa
Calculated molecular weight
71607 MW
Background of PRC1
Protein Regulator of cytokinesis 1 (PRC1) is a protein that in humans is encoded by the PRC1 gene and is involved in cytokinesis. This gene is mapped to chromosome 15q26.1 based on an alignment of the PRC1 sequence with the genomic sequence. PRC1 protein is expressed at relatively high levels during S and G2/M phases of the cell cycle before dropping dramatically after mitotic exit and entrance into G1 phase. It is located in the nucleus during interphase, becomes associated with the mitotic spindle in a highly dynamic manner during anaphase, and localizes to the cell midbody during cytokinesis. And this protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9814 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: MCF-7 Whole Cell, SGC Whole Cell, HELA Whole Cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of PRC1 using anti-PRC1 antibody (PB9814).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions.
Lane 1: MCF-7 Whole Cell Lysate,
Lane 2: SGC Whole Cell Lysate,
Lane 3: HELA Whole Cell Lysate.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 antigen affinity purified polyclonal antibody (Catalog # PB9814) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PRC1 at approximately 75 kDa, 80 kDa. The expected band size for PRC1 is at 75 kDa, 80 kDa.
Protein Target Info & Infographic
Gene/Protein Information For PRC1 (Source: Uniprot.org, NCBI)
Gene Name
PRC1
Full Name
Protein regulator of cytokinesis 1
Weight
71607 MW
Superfamily
MAP65/ASE1 family
Alternative Names
Protein regulator of cytokinesis 1 ;PRC1 ; PRC1 ASE1 protein regulator of cytokinesis 1 protein regulator of cytokinesis 1|anaphase spindle elongation 1 homolog|protein regulating cytokinesis 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PRC1, check out the PRC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PRC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PRC1 Antibody Picoband® (PB9814)
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Customer Q&As
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4 Customer Q&As for Anti-PRC1 Antibody Picoband®
Question
My colleagues were well pleased with the WB result of your anti-PRC1 antibody. However we have been able to see positive staining in cervix carcinoma nucleus. using this antibody. Is that expected? Could you tell me where is PRC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-05-05
Answer
From what I have seen in literature, cervix carcinoma does express PRC1. Generally PRC1 expresses in nucleus. Regarding which tissues have PRC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-05-05
Question
We are currently using anti-PRC1 antibody PB9814 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
The anti-PRC1 antibody (PB9814) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-29
Question
We have observed staining in human cervix carcinoma erythroleukemia. Are there any suggestions? Is anti-PRC1 antibody supposed to stain cervix carcinoma erythroleukemia positively?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
From what I have seen in literature cervix carcinoma erythroleukemia does express PRC1. From what I have seen in Uniprot.org, PRC1 is expressed in testis, kidney eco:0000312, embl:aah03138.1
rc placenta eco:0000312, embl:aah05140.1, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have PRC1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Kidney, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-26
Question
you antibody using your anti-PRC1 antibody for mitotic spindle elongation studies. Has this antibody been tested with western blotting on sgc whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
Thank you for your inquiry. This PB9814 anti-PRC1 antibody is tested on sgc whole cell lysate, hela whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-01-09
