Product Info Summary
SKU: | A17066-1 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, ICC, WB |
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Product info
Product Name
Anti-PPP1R36 Antibody Picoband™
SKU/Catalog Number
A17066-1
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-PPP1R36 Antibody Picoband™ catalog # A17066-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-PPP1R36 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A17066-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human PPP1R36 recombinant protein (Position: R44-D381).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A17066-1 is reactive to PPP1R36 in Human
Applications
A17066-1 is guaranteed for ELISA, Flow Cytometry, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
54 kDa
Calculated molecular weight
24145 MW
Background of PPP1R36
PPP1R36 (protein phosphatase 1, regulatory subunit 36) is a protein-coding gene. GO annotations related to this gene include phosphatase binding and protein phosphatase inhibitor activity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x1x106 cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of PPP1R36 using anti-PPP1R36 antibody (A17066-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human SIHA whole cell lysates,
Lane 3: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R36 antigen affinity purified polyclonal antibody (Catalog # A17066-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP1R36 at approximately 54 kDa. The expected band size for PPP1R36 is at 49 kDa.
Click image to see more details
Figure 2. IF analysis of PPP1R36 using anti-PPP1R36 antibody (A17066-1) and anti-Tubulin Alpha antibody (M03989-3).
PPP1R36 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-PPP1R36 Antibody (A17066-1) and mouse anti-Tubulin Alpha antibody (M03989-3) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) and DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 3. Flow Cytometry analysis of Hela cells using anti-PPP1R36 antibody (A17066-1).
Overlay histogram showing Hela cells stained with A17066-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPP1R36 Antibody (A17066-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For PPP1R36 (Source: Uniprot.org, NCBI)
Gene Name
PPP1R36
Full Name
Protein phosphatase 1 regulatory subunit 36
Weight
24145 MW
Alternative Names
C14orf50; chromosome 14 open reading frame 50; hypothetical protein LOC145376 PPP1R36 C14orf50 protein phosphatase 1 regulatory subunit 36 protein phosphatase 1 regulatory subunit 36
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPP1R36, check out the PPP1R36 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPP1R36: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PPP1R36 Antibody Picoband™ (A17066-1)
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