Anti-PPP1R14D/Gbpi 1 Antibody

PPP1R14D/GBPI-1 antibody

Boster Bio Anti-PPP1R14D/Gbpi 1 Antibody catalog # A14433. Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A14433
Size: 100μl
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-PPP1R14D/Gbpi 1 Antibody

View all PPP1R14D/GBPI-1 Antibodies

SKU/Catalog Number

A14433

Size

100μl

Form

Liquid

Description

Boster Bio Anti-PPP1R14D/Gbpi 1 Antibody catalog # A14433. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-PPP1R14D/Gbpi 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14433)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human PPP1R14D. at AA rangle: 40-120

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A14433 is reactive to PPP1R14D in Human

Applications

A14433 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

16508 MW

Background of PPP1R14D/GBPI-1

RNA binding motif protein 47 is a protein in humans that is encoded by the RBM47 gene in chromosome 4. RBM47 may be involved in RNA binding and nucleotide binding.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For PPP1R14D (Source: Uniprot.org, NCBI)

Gene Name

PPP1R14D

Full Name

Protein phosphatase 1 regulatory subunit 14D

Weight

16508 MW

Superfamily

PP1 inhibitor family

Alternative Names

CPI17-like; FLJ20251; gastrointestinal and brain-specific PP1-inhibitory protein 1; GBPI; GBPI1; GBPI-1; gut and brain phosphatase inhibitor 1; MGC119014; MGC119016; PKC-dependent PP1 inhibitory protein; protein phosphatase 1 regulatory subunit 14D; protein phosphatase 1, regulatory (inhibitor) subunit 14D PPP1R14D CPI17-like, GBPI-1, GBPI1 protein phosphatase 1 regulatory inhibitor subunit 14D protein phosphatase 1 regulatory subunit 14D|PKC-dependent PP1 inhibitory protein|gastrointestinal and brain-specific PP1-inhibitory protein 1|gut and brain phosphatase inhibitor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on PPP1R14D, check out the PPP1R14D Infographic

PPP1R14D infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for PPP1R14D: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-PPP1R14D/Gbpi 1 Antibody

Question

Here is the WB image, lot number and protocol we used for mucosa of transverse colon using anti-PPP1R14D/Gbpi 1 antibody A14433. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-04-23

Question

We are currently using anti-PPP1R14D/Gbpi 1 antibody A14433 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?

L. Jones

Verified customer

Asked: 2020-04-08

Answer

The anti-PPP1R14D/Gbpi 1 antibody (A14433) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-08

Question

I have a question about product A14433, anti-PPP1R14D/Gbpi 1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-26

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14433 anti-PPP1R14D/Gbpi 1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-26

Question

Does anti-PPP1R14D/Gbpi 1 antibody A14433 work for WB with mucosa of transverse colon?

G. Anderson

Verified customer

Asked: 2018-11-16

Answer

According to the expression profile of mucosa of transverse colon, PPP1R14D is highly expressed in mucosa of transverse colon. So, it is likely that anti-PPP1R14D/Gbpi 1 antibody A14433 will work for WB with mucosa of transverse colon.

Boster Scientific Support

Answered: 2018-11-16

Order DetailsPrice
A14433

100uL

$399

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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