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Product Info Summary
| SKU: | A02536-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Dynamin 1 DyLight® 488 conjugated DNM1 Antibody
SKU/Catalog Number
A02536-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Dynamin 1 DyLight® 488 conjugated DNM1 Antibody catalog # A02536-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Dynamin 1 DyLight® 488 conjugated DNM1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02536-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Dynamin 1 recombinant protein (Position: W616-D667).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A02536-Dyl488 is reactive to DNM1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
97.408kDa
Background of Dynamin
Dynamin-1 is a protein that in humans is encoded by the DNM1 gene. This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02536-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Validation Images & Assay Conditions
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Boster Kit Box
Protein Target Info & Infographic
Gene/Protein Information For DNM1 (Source: Uniprot.org, NCBI)
Gene Name
DNM1
Full Name
Dynamin-1
Weight
97.408kDa
Superfamily
TRAFAC class dynamin-like GTPase superfamily
Alternative Names
Dynamin-1; DNM1; DNM DNM1 DEE31, DNM, EIEE31 dynamin 1 dynamin-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNM1, check out the DNM1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Dynamin 1 DyLight® 488 conjugated DNM1 Antibody (A02536-Dyl488)
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No publications found for A02536-Dyl488
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Dynamin 1 DyLight® 488 conjugated DNM1 Antibody
Question
We are currently using anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 for human tissue, and we are happy with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-Human Dynamin 1 DyLight® 488 conjugated antibody (A02536-Dyl488) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-Human Dynamin 1 DyLight® 488 conjugated antibody (A02536-Dyl488)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
We do provide the blocking peptide for product anti-Human Dynamin 1 DyLight® 488 conjugated antibody (A02536-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-07
Question
I was wanting to use your anti-Human Dynamin 1 DyLight® 488 conjugated antibody for Flow Cytometry for human brain pns on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain pns identification?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
It shows on the product datasheet, A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain pns in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-30
Question
Is this A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody reactive to the isotypes of DNM1?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
The immunogen of A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody is E. coli-derived human Dynamin 1 recombinant protein (Position: W616-D667). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-27
Question
We have seen staining in human brain pns. Are there any suggestions? Is anti-Human Dynamin 1 DyLight® 488 conjugated antibody supposed to stain brain pns positively?
Verified Customer
Verified customer
Asked: 2019-08-13
Answer
Based on literature brain pns does express DNM1. Based on Uniprot.org, DNM1 is expressed in frontal cortex, brain pns, cervix carcinoma, platelet, among other tissues. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-08-13
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain pns using anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-23
Question
My question regarding product A02536-Dyl488, anti-Human Dynamin 1 DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-06-19
Question
See below the WB image, lot number and protocol we used for brain pns using anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488. Please let me know if you require anything else.
D. Anderson
Verified customer
Asked: 2018-11-14
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-14
Question
Will anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 work for Flow Cytometry with brain pns?
Verified Customer
Verified customer
Asked: 2018-10-19
Answer
According to the expression profile of brain pns, DNM1 is highly expressed in brain pns. So, it is likely that anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 will work for Flow Cytometry with brain pns.
Boster Scientific Support
Answered: 2018-10-19
Question
We were content with the WB result of your anti-Human Dynamin 1 DyLight® 488 conjugated antibody. However we have seen positive staining in cervix carcinoma cytoplasm. using this antibody. Is that expected? Could you tell me where is DNM1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
Based on literature, cervix carcinoma does express DNM1. Generally DNM1 expresses in cytoplasm. Regarding which tissues have DNM1 expression, here are a few articles citing expression in various tissues:
Brain, and PNS, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2017-10-26
Question
I see that the anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-10-09
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-10-09
Question
My lab would like to test anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 on human brain pns for research purposes, then I may be interested in using anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Bhatt
Verified customer
Asked: 2017-10-04
Answer
The products we sell, including anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-04
Question
Does A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Carter
Verified customer
Asked: 2017-07-03
Answer
As indicated on the product datasheet, A02536-Dyl488 anti-Human Dynamin 1 DyLight® 488 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-03
Question
Is there a BSA free version of anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 available?
T. Jackson
Verified customer
Asked: 2016-01-07
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Human Dynamin 1 DyLight® 488 conjugated antibody A02536-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-01-07
