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Product Info Summary
| SKU: | A11054-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-PP2C epsilon PPM1L Antibody
View all PP2C epsilon/PPM1L Antibodies
SKU/Catalog Number
A11054-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-PP2C epsilon PPM1L Antibody catalog # A11054-1. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PP2C epsilon PPM1L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11054-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human PPM1L. AA range:119-168
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11054-1 is reactive to PPM1L in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
41053 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11054-1 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of Jurkat cells using PP2Cepsilon Polyclonal antibody.
Protein Target Info & Infographic
Gene/Protein Information For PPM1L (Source: Uniprot.org, NCBI)
Gene Name
PPM1L
Full Name
Protein phosphatase 1L
Weight
41053 MW
Superfamily
PP2C family
Alternative Names
Protein phosphatase 1L;3.1.3.16;Protein phosphatase 1-like;Protein phosphatase 2C isoform epsilon;PP2C-epsilon;PPM1L;PP2CE; PPM1L PP2C-epsilon, PP2CE, PPM1-LIKE protein phosphatase, Mg2+/Mn2+ dependent 1L protein phosphatase 1L|PP2C epsilon
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PPM1L, check out the PPM1L Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PPM1L: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PP2C epsilon PPM1L Antibody (A11054-1)
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No publications found for A11054-1
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-PP2C epsilon PPM1L Antibody
Question
Is a blocking peptide available for product anti-PP2C epsilon antibody (A11054-1)?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
We do provide the blocking peptide for product anti-PP2C epsilon antibody (A11054-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-03-24
Question
We are currently using anti-PP2C epsilon antibody A11054-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The anti-PP2C epsilon antibody (A11054-1) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-27
Question
I have a question about product A11054-1, anti-PP2C epsilon antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11054-1 anti-PP2C epsilon antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-31
Question
Will anti-PP2C epsilon antibody A11054-1 work on bovine WB with layer of synovial tissue?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Our lab technicians have not validated anti-PP2C epsilon antibody A11054-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-PP2C epsilon antibody A11054-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine layer of synovial tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-21
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-PP2C epsilon antibody A11054-1. Let me know if you need anything else.
N. Krishna
Verified customer
Asked: 2018-12-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-03
Question
Will A11054-1 anti-PP2C epsilon antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-19
Answer
As indicated on the product datasheet, A11054-1 anti-PP2C epsilon antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-19
