Product Info Summary
SKU: | A11197 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-CAPN9/Calpain 9 Antibody
SKU/Catalog Number
A11197
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CAPN9/Calpain 9 Antibody (Catalog # A11197). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CAPN9/Calpain 9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11197)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CAPN9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11197 is reactive to CAPN9 in Human
Reconstitution
Calculated molecular weight
79097 MW
Background of Calpain 9
Calcium-regulated non-lysosomal thiol-protease.
Lee H.-J., Biol. Chem. 379:175-183(1998).
Yoshikawa Y., Jpn. J. Cancer Res. 91:459-463(2000).
Gregory S.G., Nature 441:315-321(2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11197 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from HuvEc cells, using CAPN9 antibody A11197.
Protein Target Info & Infographic
Gene/Protein Information For CAPN9 (Source: Uniprot.org, NCBI)
Gene Name
CAPN9
Full Name
Calpain-9
Weight
79097 MW
Superfamily
peptidase C2 family
Alternative Names
Calpain-9;3.4.22.-;Digestive tract-specific calpain;New calpain 4;nCL-4;Protein CG36;CAPN9;NCL4; CAPN9 GC36, nCL-4 calpain 9 calpain-9|digestive tract-specific calpain|new calpain 4|novel calpain large subunit-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CAPN9, check out the CAPN9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CAPN9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CAPN9/Calpain 9 Antibody (A11197)
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No publications found for A11197
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CAPN9/Calpain 9 Antibody
Question
Do you have a BSA free version of anti-CAPN9/Calpain 9 antibody A11197 available?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CAPN9/Calpain 9 antibody A11197 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-20
Question
Is a blocking peptide available for product anti-CAPN9/Calpain 9 antibody (A11197)?
R. Edwards
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-CAPN9/Calpain 9 antibody (A11197). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
My question regarding product A11197, anti-CAPN9/Calpain 9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-03-19
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11197 anti-CAPN9/Calpain 9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-03-19
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-CAPN9/Calpain 9 antibody A11197. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-11-30
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-30
Question
I was wanting to use your anti-CAPN9/Calpain 9 antibody for WB for human stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human stomach identification?
D. Carter
Verified customer
Asked: 2017-03-06
Answer
You can see on the product datasheet, A11197 anti-CAPN9/Calpain 9 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human stomach in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-03-06
Question
We are currently using anti-CAPN9/Calpain 9 antibody A11197 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?
B. Mitchell
Verified customer
Asked: 2015-07-10
Answer
The anti-CAPN9/Calpain 9 antibody (A11197) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-07-10
Question
Here is the WB image, lot number and protocol we used for stomach using anti-CAPN9/Calpain 9 antibody A11197. Please let me know if you require anything else.
A. Anderson
Verified customer
Asked: 2014-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-24