Anti-CAPN9/Calpain 9 Antibody

Calpain 9 antibody

Boster Bio Anti-CAPN9/Calpain 9 Antibody (Catalog # A11197). Tested in WB applications. This antibody reacts with Human.

Product Info Summary

SKU: A11197
Size: 100 µl
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-CAPN9/Calpain 9 Antibody

View all Calpain 9 Antibodies

SKU/Catalog Number

A11197

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-CAPN9/Calpain 9 Antibody (Catalog # A11197). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CAPN9/Calpain 9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11197)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human CAPN9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A11197 is reactive to CAPN9 in Human

Reconstitution

Calculated molecular weight

79097 MW

Background of Calpain 9

Calcium-regulated non-lysosomal thiol-protease.

Lee H.-J., Biol. Chem. 379:175-183(1998).
Yoshikawa Y., Jpn. J. Cancer Res. 91:459-463(2000).
Gregory S.G., Nature 441:315-321(2006).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A11197 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For CAPN9 (Source: Uniprot.org, NCBI)

Gene Name

CAPN9

Full Name

Calpain-9

Weight

79097 MW

Superfamily

peptidase C2 family

Alternative Names

Calpain-9;3.4.22.-;Digestive tract-specific calpain;New calpain 4;nCL-4;Protein CG36;CAPN9;NCL4; CAPN9 GC36, nCL-4 calpain 9 calpain-9|digestive tract-specific calpain|new calpain 4|novel calpain large subunit-4

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CAPN9, check out the CAPN9 Infographic

CAPN9 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CAPN9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-CAPN9/Calpain 9 Antibody

Question

Do you have a BSA free version of anti-CAPN9/Calpain 9 antibody A11197 available?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CAPN9/Calpain 9 antibody A11197 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-20

Question

Is a blocking peptide available for product anti-CAPN9/Calpain 9 antibody (A11197)?

R. Edwards

Verified customer

Asked: 2019-08-20

Answer

We do provide the blocking peptide for product anti-CAPN9/Calpain 9 antibody (A11197). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-20

Question

My question regarding product A11197, anti-CAPN9/Calpain 9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2018-03-19

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11197 anti-CAPN9/Calpain 9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2018-03-19

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for stomach using anti-CAPN9/Calpain 9 antibody A11197. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-11-30

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-11-30

Question

I was wanting to use your anti-CAPN9/Calpain 9 antibody for WB for human stomach on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human stomach identification?

D. Carter

Verified customer

Asked: 2017-03-06

Answer

You can see on the product datasheet, A11197 anti-CAPN9/Calpain 9 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human stomach in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-03-06

Question

We are currently using anti-CAPN9/Calpain 9 antibody A11197 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on horse tissues as well?

B. Mitchell

Verified customer

Asked: 2015-07-10

Answer

The anti-CAPN9/Calpain 9 antibody (A11197) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-07-10

Question

Here is the WB image, lot number and protocol we used for stomach using anti-CAPN9/Calpain 9 antibody A11197. Please let me know if you require anything else.

A. Anderson

Verified customer

Asked: 2014-12-24

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-12-24

Order DetailsPrice
A11197-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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