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Product Info Summary
| SKU: | M00305-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-POMC Monoclonal Antibody
SKU/Catalog Number
M00305-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-POMC Monoclonal Antibody catalog # M00305-1. Tested in WB, IHC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POMC Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00305-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADEC-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human POMC ACTH stimulates the adrenal glands to release cortisol. MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin and Met-enkephalin are endogenous opiates.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00305-1 is reactive to POMC in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
62 kDa
Calculated molecular weight
39830 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00305-1 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:500
IP 1:50
Positive Control
WB: mouse pituitary
Validation Images & Assay Conditions
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Western blot analysis of POMC expression in mouse pituitary lysate.
Protein Target Info & Infographic
Gene/Protein Information For POMC (Source: Uniprot.org, NCBI)
Gene Name
POMC
Full Name
Pro-opiomelanocortin
Weight
39830 MW
Superfamily
POMC family
Alternative Names
Vasodilator-stimulated phosphoprotein;VASP;VASP; POMC ACTH, CLIP, LPH, MSH, NPP, OBAIRH, POC proopiomelanocortin pro-opiomelanocortin|adrenocorticotropic hormone|adrenocorticotropin|alpha-MSH|alpha-melanocyte-stimulating hormone|beta-LPH|beta-MSH|beta-endorphin|beta-melanocyte-stimulating hormone|corticotropin-like intermediary peptide|corticotropin-lipotropin|gamma-LPH|gamma-MSH|lipotropin beta|lipotropin gamma|melanotropin alpha|melanotropin beta|melanotropin gamma|met-enkephalin|opiomelanocortin prepropeptide|pro-ACTH-endorphin|proopiomelanocortin preproprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POMC, check out the POMC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POMC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POMC Monoclonal Antibody (M00305-1)
Hello CJ!
M00305-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Huo J, Liu J, Wang J, Zhang Y, Wang C, Yang Y, Sun W, Xu S. Neural Regen Res. 2012 Aug 5;7(22):1729-35. Doi: 10.3969/J.Issn.1673-5374.2012.22.008. Early Hyperbaric Oxygen Therapy Inhibits Aquaporin 4 And Adrenocorticotropic Hormone Expression In T...
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Customer Q&As
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4 Customer Q&As for Anti-POMC Monoclonal Antibody
Question
Our lab used your anti-POMC Monoclonal antibody for WB on pituitary in the past. I am using mouse, and I plan to use the antibody for IP next. We want examining pituitary as well as pituitary gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
D. Edwards
Verified customer
Asked: 2020-02-25
Answer
I viewed the website and datasheets of our anti-POMC Monoclonal antibody and it appears that M00305-1 has been tested on mouse in both WB and IP. Thus M00305-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-02-25
Question
We have seen staining in mouse pituitary gland. Do you have any suggestions? Is anti-POMC Monoclonal antibody supposed to stain pituitary gland positively?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
According to literature pituitary gland does express POMC. According to Uniprot.org, POMC is expressed in pituitary gland, pituitary, among other tissues. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2019-10-18
Question
We are currently using anti-POMC Monoclonal antibody M00305-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-03
Answer
The anti-POMC Monoclonal antibody (M00305-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-03
Question
We were satisfied with the WB result of your anti-POMC Monoclonal antibody. However we have been able to see positive staining in pituitary gland secreted. using this antibody. Is that expected? Could you tell me where is POMC supposed to be expressed?
K. Singh
Verified customer
Asked: 2017-07-24
Answer
From what I have seen in literature, pituitary gland does express POMC. Generally POMC expresses in secreted. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2017-07-24
