Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody

Product Name

Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody

View all POMC Antibodies

SKU/Catalog Number

M00305

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody (Catalog # M00305). Tested in ELISA, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) (Pituitary Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00305)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: r57

Isotype

IgG1, kappa

Immunogen

N-terminal fragment of human ACTH conjugated to KLH

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00305 is reactive to POMC in Human, Mouse, Rat

Applications

M00305 is guaranteed for ELISA, IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

29424 MW

Background of POMC

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This monoclonal antibody is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs).It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with 1ml water (15 minutes at room temperature).

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA (For coating, order antibody without BSA)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human pituitary gland stained with Anti-ACTH Recombinant Mouse Monoclonal Antibody (r57).

Gene/Protein Information For POMC (Source: Uniprot.org, NCBI)

Alternative Names

ACTH; adrenocorticotropic hormone; adrenocorticotropin; alpha-melanocyte-stimulating hormone; alpha-MSH; beta-endorphin; beta-LPH; beta-melanocyte-stimulating hormone; beta-MSH; CLIP; corticotropin-like intermediary peptide; corticotropin-lipotropin; gamma-LPH; gamma-MSH; lipotropin beta; lipotropin gamma; LPH; melanotropin alpha; melanotropin beta; melanotropin gamma; met-enkephalin; MSH; NPP; POC; POMC; pro-ACTH-endorphin; proopiomelanocortin preproprotein; proopiomelanocortin; pro-opiomelanocortin POMC ACTH, CLIP, LPH, MSH, NPP, OBAIRH, POC proopiomelanocortin pro-opiomelanocortin|adrenocorticotropic hormone|adrenocorticotropin|alpha-MSH|alpha-melanocyte-stimulating hormone|beta-LPH|beta-MSH|beta-endorphin|beta-melanocyte-stimulating hormone|corticotropin-like intermediary peptide|corticotropin-lipotropin|gamma-LPH|gamma-MSH|lipotropin beta|lipotropin gamma|melanotropin alpha|melanotropin beta|melanotropin gamma|met-enkephalin|opiomelanocortin prepropeptide|pro-ACTH-endorphin|proopiomelanocortin preproprotein

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