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Product Info Summary
| SKU: | PB9039 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-ACTH/POMC Antibody
SKU/Catalog Number
PB9039
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ACTH/POMC Antibody catalog # PB9039. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTH/POMC Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9039)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human ACTH, different from the related mouse and rat sequences by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9039 is reactive to POMC in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
29424 MW
Background of POMC
Adrenocorticotropic hormone (ACTH), also known as corticotropin, is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with its precursor corticotropin-releasing hormone from the hypothalamus). Its principal effects are increased production and release of corticosteroids. ACTH stimulates secretion of glucocorticoid steroid hormones from adrenal cortex cells, especially in the zona fasciculata of the adrenal glands. This gene can influence steroid hormone secretion by both rapid short-term mechanisms that take place within minutes and slower long-term actions. Besides, ACTH also enhances transcription of mitochondrial genes that encode for subunits of mitochondrial oxidative phosphorylation systems.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9039 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Positive Control
IHC: mouse kidney tissue, rat brain tissue, rat kidney tissue
Validation Images & Assay Conditions
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Figure 1. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of mouse kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of rat brain tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of ACTH using anti-ACTH antibody (PB9039). ACTH was detected in paraffin-embedded section of rat kidney tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ACTH Antibody (PB9039) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For POMC (Source: Uniprot.org, NCBI)
Gene Name
POMC
Full Name
Pro-opiomelanocortin
Weight
29424 MW
Superfamily
POMC family
Alternative Names
Pro-opiomelanocortin;POMC;Corticotropin-lipotropin;NPP;Melanotropin gamma;Gamma-MSH;Potential peptide;Corticotropin;Adrenocorticotropic hormone;ACTH;Melanotropin alpha;Alpha-MSH;Corticotropin-like intermediary peptide;CLIP;Lipotropin beta;Beta-LPH;Lipotropin gamma;Gamma-LPH;Melanotropin beta;Beta-MSH;Beta-endorphin;Met-enkephalin;POMC; POMC ACTH, CLIP, LPH, MSH, NPP, OBAIRH, POC proopiomelanocortin pro-opiomelanocortin|adrenocorticotropic hormone|adrenocorticotropin|alpha-MSH|alpha-melanocyte-stimulating hormone|beta-LPH|beta-MSH|beta-endorphin|beta-melanocyte-stimulating hormone|corticotropin-like intermediary peptide|corticotropin-lipotropin|gamma-LPH|gamma-MSH|lipotropin beta|lipotropin gamma|melanotropin alpha|melanotropin beta|melanotropin gamma|met-enkephalin|opiomelanocortin prepropeptide|pro-ACTH-endorphin|proopiomelanocortin preproprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POMC, check out the POMC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POMC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ACTH/POMC Antibody (PB9039)
Hello CJ!
PB9039 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen L, Wang H, Zhao Z, Zhang Y, Huang G. Am J Chin Med. 2005;33(6):945-55. Effects Of The Extract Of A Chinese Herb Tripterygium Wilfordii Hook F On Rat Pituitary Gland.
Huo J, Liu J, Wang J, Zhang Y, Wang C, Yang Y, Sun W, Xu S. Neural Regen Res. 2012 Aug 5;7(22):1729-35. Doi: 10.3969/J.Issn.1673-5374.2012.22.008. Early Hyperbaric Oxygen Therapy Inhibits Aquaporin 4 And Adrenocorticotropic Hormone Expression In T...
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4 Customer Q&As for Anti-ACTH/POMC Antibody
Question
We are currently using anti-ACTH/POMC antibody PB9039 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The anti-ACTH/POMC antibody (PB9039) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-04
Question
We have been able to see staining in mouse pituitary. Do you have any suggestions? Is anti-ACTH/POMC antibody supposed to stain pituitary positively?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
From what I have seen in literature pituitary does express POMC. From what I have seen in Uniprot.org, POMC is expressed in pituitary gland, pituitary, among other tissues. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2020-01-06
Question
My lab would like using your anti-ACTH/POMC antibody for antimicrobial humoral immune response mediated by antimicrobial peptide studies. Has this antibody been tested with western blotting on brain tissue? We would like to see some validation images before ordering.
J. Zhao
Verified customer
Asked: 2019-12-31
Answer
We appreciate your inquiry. This PB9039 anti-ACTH/POMC antibody is validated on mouse kidney tissue, rat kidney tissue, brain tissue. It is guaranteed to work for IHC in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-31
Question
We were well pleased with the WB result of your anti-ACTH/POMC antibody. However we have been able to see positive staining in pituitary gland secreted. using this antibody. Is that expected? Could you tell me where is POMC supposed to be expressed?
N. Wu
Verified customer
Asked: 2014-06-27
Answer
According to literature, pituitary gland does express POMC. Generally POMC expresses in secreted. Regarding which tissues have POMC expression, here are a few articles citing expression in various tissues:
Pituitary, Pubmed ID: 7828531, 15489334, 16807684
Boster Scientific Support
Answered: 2014-06-27
