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Product Info Summary
| SKU: | A01029S2 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-POLR2A (Phospho-S2) Antibody
View all RNA Polymerase II/POLR2A Antibodies
SKU/Catalog Number
A01029S2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLR2A (Phospho-S2) Antibody catalog # A01029S2. Tested in WB,IP,ICC/IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLR2A (Phospho-S2) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01029S2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding Ser2 of human POLR2A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A01029S2 is reactive to POLR2A in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
217176 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01029S2 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of POLR2A using Anti-POLR2A (Phospho-S2) Antibody (A01029S2).
ICC staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-POLR2A (Phospho-S2) Antibody A01029S2
Western blot analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti-Phospho-POLR2A (S2) antibody at 1/500 dilution. Positive control：Line 1: Hela
Line 2: MCF-7
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For POLR2A (Source: Uniprot.org, NCBI)
Gene Name
POLR2A
Full Name
DNA-directed RNA polymerase II subunit RPB1
Weight
217176 MW
Superfamily
RNA polymerase beta' chain family
Alternative Names
DNA-directed RNA polymerase II subunit RPB1;RNA polymerase II subunit B1;2.7.7.6;DNA-directed RNA polymerase II subunit A;DNA-directed RNA polymerase III largest subunit;RNA-directed RNA polymerase II subunit RPB1;2.7.7.48;POLR2A;POLR2; POLR2A NEDHIB, POLR2, POLRA, RPB1, RPBh1, RPO2, RPOL2, RpIILS, hRPB220, hsRPB1 RNA polymerase II subunit A DNA-directed RNA polymerase II subunit RPB1|DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit|DNA-directed RNA polymerase II subunit A|DNA-directed RNA polymerase III largest subunit|RNA polymerase II subunit B1|RNA-directed RNA polymerase II subunit RPB1|polymerase (RNA) II (DNA directed) polypeptide A, 220kDa|polymerase (RNA) II subunit A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLR2A, check out the POLR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLR2A (Phospho-S2) Antibody (A01029S2)
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