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Product Info Summary
| SKU: | A30645 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-POLE4 Antibody
SKU/Catalog Number
A30645
Size
100ul
Form
Liquid
Description
Boster Bio Anti-POLE4 Antibody (Catalog# A30645). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-POLE4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30645)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human POLE4. Immunogen sequence location: 61-110
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30645 is reactive to POLE4 in Human, Mouse
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
12209 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30645 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using POLE4 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For POLE4 (Source: Uniprot.org, NCBI)
Gene Name
POLE4
Full Name
DNA polymerase epsilon subunit 4
Weight
12209 MW
Alternative Names
POLE4;DNA polymerase epsilon subunit 4;DNA polymerase II subunit 4;DNA polymerase epsilon subunit p12 POLE4 YHHQ1, p12 DNA polymerase epsilon 4, accessory subunit DNA polymerase epsilon subunit 4|DNA polymerase II subunit 4|DNA polymerase epsilon subunit p12|polymerase (DNA) epsilon 4, accessory subunit|polymerase (DNA-directed), epsilon 4, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLE4, check out the POLE4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLE4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLE4 Antibody (A30645)
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No publications found for A30645
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Customer Q&As
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1 Customer Q&As for Anti-POLE4 Antibody
Question
We are currently using anti-POLE4 antibody A30645 for mouse tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on horse tissues as well?
E. Thomas
Verified customer
Asked: 2019-10-01
Answer
The anti-POLE4 antibody (A30645) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-01
