Product Info Summary
SKU: | A09182 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DNA pol Epsilon 2 POLE2 Antibody
View all DNA Polymerase epsilon p59 Antibodies
SKU/Catalog Number
A09182
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA pol Epsilon 2 POLE2 Antibody catalog # A09182. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA pol Epsilon 2 POLE2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09182)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CDK5 Activator-binding C42
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09182 is reactive to POLE2 in Human, Mouse, Rat
Applications
A09182 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
59537 MW
Background of DNA Polymerase epsilon p59
Required for synapse integrity and synaptic plasticity. During cerebellar synapse formation, essential for the formation and maintenance of parallel fiber and Purkinje cell synapses. When parallel fibers make contact with Purkinje spines, CBLN1 interaction with GRID2 triggers the recruitment of NRXN1 and secretory vesicles to the sites of contact. NRXN1-CBLN1-GRID2 signaling induces presynaptic morphological changes, which may further accumulate pre- and postsynaptic components to promote bidirectional maturation of parallel fiber - Purkinje cell functionnally active synapses by a positive feedback mechanism. Required for CBLN3 export from the endoplasmic reticulum and secretion By similarity.The cerebellin peptide exerts neuromodulatory functions. Directly stimulates norepinephrine release via the adenylate cyclase/PKA-dependent signaling pathway; and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release By similarity.
Urade Y., Proc. Natl. Acad. Sci. U.S.A. 88:1069-1073(1991).
Ota T., Nat. Genet. 36:40-45(2004).
Mural R.J., Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1000
Validation Images & Assay Conditions
![a09182 anti dna pol epsilon 2 pole2 antibody wb testing 1 a09182 anti dna pol epsilon 2 pole2 antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a09182-anti-dna-pol-epsilon-2-pole2-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-DNA pol Epsilon 2 POLE2 Antibody A09182
Western blot (WB) analysis of DNA pol ε 2 polyclonal antibody at 1:500 dilutionLane1:MCF-7 cell lysate
Lane2:sp2/0 cell lysate
Lane3:PC12 cell lysate
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For POLE2 (Source: Uniprot.org, NCBI)
Gene Name
POLE2
Full Name
DNA polymerase epsilon subunit 2
Weight
59537 MW
Superfamily
DNA polymerase epsilon subunit B family
Alternative Names
DNA polymerase epsilon subunit 2; DNA polymerase II subunit 2; DPE2DNA polymerase epsilon subunit B; EC 2.7.7.7; polymerase (DNA directed), epsilon 2 (p59 subunit) POLE2 DPE2 DNA polymerase epsilon 2, accessory subunit DNA polymerase epsilon subunit 2|DNA polymerase II subunit 2|DNA polymerase epsilon subunit B|polymerase (DNA directed), epsilon 2 (p59 subunit)|polymerase (DNA directed), epsilon 2, accessory subunit|polymerase (DNA) epsilon 2, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLE2, check out the POLE2 Infographic
![POLE2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLE2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA pol Epsilon 2 POLE2 Antibody (A09182)
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No publications found for A09182
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