Product Info Summary
SKU: | A01946-2 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat, Zebrafish |
Host: | Rabbit |
Application: | IP, IHC, ICC, WB |
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Product info
Product Name
Anti-DNA Polymerase Beta POLB Antibody
View all DNA Polymerase beta Antibodies
SKU/Catalog Number
A01946-2
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DNA Polymerase Beta POLB Antibody catalog # A01946-2. Tested in WB,IHC,IP,ICC applications. This antibody reacts with Human,Mouse,Rat,Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DNA Polymerase Beta POLB Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01946-2)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human HtrA2 around the phosphorylation site of S212.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01946-2 is reactive to POLB in Human, Mouse, Rat, Zebrafish
Applications
A01946-2 is guaranteed for IP, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38178 MW
Background of DNA Polymerase beta
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 5 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 5 seems to be involved proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. Cullin 5 may form a cell surface vasopressin receptor. Cullin 5 is part of a E3 ubiquitin ligase complex with elongin BC complex (TCEB1 and TCEB2), RBX1 and MUF1, complexes with elongin BC complex (TCEB1 and TCEB2), RXB1 and TCEB3 or SOCS1 or WSB1; elongin BC complex (TCEB1 and TCEB2), RBX1 and VHL; elongin BC complex (TCEB1 and TCEB2), RBX1, adenovirus type 5 E1B-55kDa protein and adenovirus type 5 E4-orf6. Cullin 5 Interacts with RBX1 and RNF7.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500
IHC: 1:50-1:200
ICC: 1:20-1:50
IP: 1:10-1:20
Validation Images & Assay Conditions
![a01946 2 anti dna polymerase beta polb antibody ihc testing 2 a01946 2 anti dna polymerase beta polb antibody ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a01946-2-anti-dna-polymerase-beta-polb-antibody-ihc-testing-2.jpg)
Click image to see more details
Figure 2. Immunohistochemistry validation of POLB using Anti-DNA Polymerase Beta POLB Antibody (A01946-2).
Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-DNA Polymerase beta antibody. Counter stained with hematoxylin.For more protocol information of IHC
![a01946 2 anti dna polymerase beta polb antibody wb testing 1 a01946 2 anti dna polymerase beta polb antibody wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a01946-2-anti-dna-polymerase-beta-polb-antibody-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blotting validation for Anti-DNA Polymerase Beta POLB Antibody A01946-2
Western blot analysis of DNA Polymerase beta on different cell lysate using anti-DNA Polymerase beta antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For POLB (Source: Uniprot.org, NCBI)
Gene Name
POLB
Full Name
DNA polymerase beta
Weight
38178 MW
Superfamily
DNA polymerase type-X family
Alternative Names
DNA pol beta; DNA polymerase beta subunit; DNA Polymerase beta; EC 2.7.7.7; EC 4.2.99.-; MGC125976; POLB; polymerase (DNA directed), beta POLB DNA polymerase beta DNA polymerase beta|DNA pol beta|DNA polymerase beta subunit|polymerase (DNA directed), beta|polymerase (DNA) beta
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLB, check out the POLB Infographic
![POLB infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DNA Polymerase Beta POLB Antibody (A01946-2)
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