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Product Info Summary
| SKU: | A12109-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-POLE3 Antibody (N-term)
View all DNA Polymerase epsilon subunit 3 Antibodies
SKU/Catalog Number
A12109-1
Size
80 µl
Description
Boster Bio Anti-POLE3 Antibody (N-term) (Catalog # A12109-1). Tested in WB, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-POLE3 Antibody (N-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12109-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This POLE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-34 amino acids from the N-terminal region of human POLE3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A12109-1 is reactive to POLE3 in Human
Applications
A12109-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
16860 Da
Background of DNA Polymerase epsilon subunit 3
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
FC: 1:10-1:50
Validation Images & Assay Conditions
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POLE3 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the POLE3 antibody detected the POLE3 protein (arrow).
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POLE3 Antibody (N-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For POLE3 (Source: Uniprot.org, NCBI)
Gene Name
POLE3
Full Name
DNA polymerase epsilon subunit 3
Weight
16860 Da
Alternative Names
Arsenic-transactivated protein; asTP; CHARAC17arsenic transactivated protein; CHRAC-17; CHRAC17Ybl1; Chromatin accessibility complex 17 kDa protein; DNA polymerase epsilon p17 subunit; DNA polymerase epsilon subunit 3; DNA polymerase epsilon subunit p17; DNA polymerase II subunit 3; EC 2.7.7.7; histone fold protein CHRAC17; huCHRAC17; p17; polymerase (DNA directed), epsilon 3 (p17 subunit); YBL1 POLE3 CHARAC17, CHRAC17, CHRAC2, YBL1, p17 DNA polymerase epsilon 3, accessory subunit DNA polymerase epsilon subunit 3|CHRAC-17|DNA polymerase II subunit 3|DNA polymerase epsilon p17 subunit|DNA polymerase epsilon subunit p17|arsenic transactivated protein|asTP|chromatin accessibility complex 17 kDa protein|chromatin accessibility complex subunit 2|histone fold protein CHRAC17|huCHRAC17|polymerase (DNA directed), epsilon 3 (p17 subunit)|polymerase (DNA directed), epsilon 3, accessory subunit|polymerase (DNA) epsilon 3, accessory subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on POLE3, check out the POLE3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for POLE3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-POLE3 Antibody (N-term) (A12109-1)
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