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Product Info Summary
| SKU: | M01262 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody
View all PMEL17/SILV Antibodies
SKU/Catalog Number
M01262
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody catalog # M01262. Tested in WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01262)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AHH-16
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human PMEL17 / GP100
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01262 is reactive to PMEL in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
100 kDa
Calculated molecular weight
70255 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01262 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
Positive Control
WB: human melanoma
IHC: human melanoma tissue
Validation Images & Assay Conditions
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Western blot analysis of PMEL17 / GP100 expression in human melanoma lysate.
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Immunohistochemical analysis of paraffin-embedded human melanoma, using PMEL17 / GP100 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For PMEL (Source: Uniprot.org, NCBI)
Gene Name
PMEL
Full Name
Melanocyte protein PMEL
Weight
70255 MW
Superfamily
PMEL/NMB family
Alternative Names
Melanocyte protein PMEL;ME20-M;ME20M;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;M-alpha;95 kDa melanocyte-specific secreted glycoprotein;P26;Secreted melanoma-associated ME20 antigen;ME20-S;ME20S;M-beta;PMEL;D12S53E, PMEL17, SILV; PMEL D12S53E, ME20, ME20-M, ME20M, P1, P10017, SI, SIL, SILV, gp100, PMEL premelanosome protein melanocyte protein PMEL|melanocyte protein Pmel 17|melanocyte protein mel 17|melanocytes lineage-specific GP100|melanoma-associated ME20 |melanosomal matrix protein17|silver locus protein homolog|silver, mouse, homolog of
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PMEL, check out the PMEL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PMEL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody (M01262)
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No publications found for M01262
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-PMEL17 / GP100 Rabbit Monoclonal Antibody
Question
Does anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 work for WB with placenta?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
According to the expression profile of placenta, PMEL is highly expressed in placenta. So, it is likely that anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 will work for WB with placenta.
Boster Scientific Support
Answered: 2020-02-03
Question
We are currently using anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The anti-PMEL17 / GP100 Rabbit Monoclonal antibody (M01262) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-15
Question
I was wanting to use your anti-PMEL17 / GP100 Rabbit Monoclonal antibody for WB for human placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta identification?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
As indicated on the product datasheet, M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-31
Question
We need to test anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 on human placenta for research purposes, then I may be interested in using anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
The products we sell, including anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-04
Question
Would M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
As indicated on the product datasheet, M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-26
Question
Is there a BSA free version of anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 available?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-01
Question
We bought anti-PMEL17 / GP100 Rabbit Monoclonal antibody for WB on placenta in the past. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining placenta as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
I looked at the website and datasheets of our anti-PMEL17 / GP100 Rabbit Monoclonal antibody and I see that M01262 has been tested on human in both WB and IHC. Thus M01262 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-24
Question
We have observed staining in human pigmented layer of retina. What should we do? Is anti-PMEL17 / GP100 Rabbit Monoclonal antibody supposed to stain pigmented layer of retina positively?
M. Williams
Verified customer
Asked: 2019-07-12
Answer
From what I have seen in literature pigmented layer of retina does express PMEL. From what I have seen in Uniprot.org, PMEL is expressed in pigmented layer of retina, placenta spleen, placenta, melanoma, among other tissues. Regarding which tissues have PMEL expression, here are a few articles citing expression in various tissues:
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 15489334
Placenta, and Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-07-12
Question
Is a blocking peptide available for product anti-PMEL17 / GP100 Rabbit Monoclonal antibody (M01262)?
Verified Customer
Verified customer
Asked: 2019-07-10
Answer
We do provide the blocking peptide for product anti-PMEL17 / GP100 Rabbit Monoclonal antibody (M01262). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-10
Question
I see that the anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262. Let me know if you need anything else.
L. Banerjee
Verified customer
Asked: 2018-02-08
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-08
Question
Is this M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody reactive to the isotypes of PMEL?
L. Thomas
Verified customer
Asked: 2017-06-21
Answer
The immunogen of M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody is A synthesized peptide derived from human PMEL17 / GP100. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-21
Question
Can you help my question with product M01262, anti-PMEL17 / GP100 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01262 anti-PMEL17 / GP100 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-05-23
Question
Here is the WB image, lot number and protocol we used for placenta using anti-PMEL17 / GP100 Rabbit Monoclonal antibody M01262. Please let me know if you require anything else.
F. Jones
Verified customer
Asked: 2015-09-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-16
Question
We were happy with the WB result of your anti-PMEL17 / GP100 Rabbit Monoclonal antibody. However we have been able to see positive staining in placenta spleen endoplasmic reticulum membrane using this antibody. Is that expected? Could you tell me where is PMEL supposed to be expressed?
J. Zhang
Verified customer
Asked: 2015-05-29
Answer
According to literature, placenta spleen does express PMEL. Generally PMEL expresses in endoplasmic reticulum membrane. Regarding which tissues have PMEL expression, here are a few articles citing expression in various tissues:
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 15489334
Placenta, and Spleen, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-05-29
