Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody

Product Name

Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody

View all PMEL17/SILV Antibodies

SKU/Catalog Number

M19774

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody (Catalog # M19774). Tested in Flow Cytometry, IF, IHC applications. This antibody reacts with Human, Horse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-gp100 / Melanosome / PMEL17 / SILV (Melanoma Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M19774)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM286

Isotype

IgG2b, kappa

Immunogen

Membranes from a human melanoma metastasis

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M19774 is reactive to SILV in Human, Horse

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

70255 MW

Background of PMEL17/SILV

By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. This monoclonal antibody reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.This monoclonal antibody labels formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M19774 is guaranteed for Flow Cytometry, IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For SILV (Source: Uniprot.org, NCBI)

Alternative Names

Melanocyte protein PMEL;ME20-M;ME20M;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;Premelanosome protein;Silver locus protein homolog;M-alpha;95 kDa melanocyte-specific secreted glycoprotein;P26;Secreted melanoma-associated ME20 antigen;ME20-S;ME20S;M-beta;PMEL;D12S53E, PMEL17, SILV; PMEL D12S53E, ME20, ME20-M, ME20M, P1, P10017, SI, SIL, SILV, gp100, PMEL premelanosome protein melanocyte protein PMEL|melanocyte protein Pmel 17|melanocyte protein mel 17|melanocytes lineage-specific GP100|melanoma-associated ME20 |melanosomal matrix protein17|silver locus protein homolog|silver, mouse, homolog of

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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